Vitrification of mouse embryos by vitroloop technique
Vitrification of mouse embryos by vitroloop technique
Fájlok
Dátum
Szerzők
Vass, Nóra
Klambauer, Philip
Jávor, András
Keresztes, Zsuzsa
Ceh, Sándor
Folyóirat címe
Folyóirat ISSN
Kötet címe (évfolyam száma)
Kiadó
Faculty of Agricultural and Food Sciences and Enviromental Management of the Debrecen University, Debrecen.
Absztrakt
Leírás
The objective of the study was to vitrify mouse embryos with the cryoloop technology using a new combination of vitrification mediums. Embryos were exposed to a 2- step loading of CPA, ethylene glycol and propylene glycol, before being placed on the surface of a thin filmy layer formed from the vitrification solution in a small nylon loop. After warming, the CPA was diluted out from the embryos by a 3-step procedure. Our data show that a high percentage of embryos survived (92.7%) vitrification in the mixture of EG and PG combined with cryoloop carrier and developing normally (89.1%) in vitro after thawing.
Kulcsszavak
vitrification, mouse embryos, vitroloop