Testing the inhibition of EGF-R by siRNA in F1-4 CHO cells

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dc.contributor.advisorBatta, Gyula
dc.contributor.authorFarhin, Syeda
dc.contributor.departmentDE--Természettudományi és Technológiai Kar--Biológiai és Ökológiai Intézet
dc.date.accessioned2024-06-10T13:40:43Z
dc.date.available2024-06-10T13:40:43Z
dc.date.created2024-04-29
dc.description.abstract- The small interfering RNA (siRNA) is a class of double-stranded, mostly exogenous RNA molecules, typically 20-25 base pairs in length, involved in post-transcriptional gene silencing. It can be used in research, but there are also a few therapeutic applications. - In our research, we investigated the inhibition of EGF-R using a predesigned siRNA in F1-4 CHO cancer cells, which express EGF-R with GFP so that it can be detected based on fluorescence. - We transfected F1-4 cells and analyzed the cells with fluorescence microscopy and flow cytometry. - Our experiments were successful, as about 30% of the cells showed low fluorescence compared to the control cells. - The successful knock down means that this siRNA can be used for further experiments in the project in the future.
dc.description.courseBiology
dc.description.degreeBSc/BA
dc.format.extent38
dc.identifier.urihttps://hdl.handle.net/2437/371310
dc.language.isoen
dc.rights.accessHozzáférhető a 2022 decemberi felsőoktatási törvénymódosítás értelmében.
dc.subjectEGF-R
dc.subjectsiRNA
dc.subjectCHO cells
dc.subjectGene silencing
dc.subjectCancer therapy
dc.subjectRNAi
dc.subjectCell signaling
dc.subject.dspaceBiology
dc.titleTesting the inhibition of EGF-R by siRNA in F1-4 CHO cells
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