Testing the inhibition of EGF-R by siRNA in F1-4 CHO cells

The small interfering RNA (siRNA) is a class of double-stranded, mostly exogenous RNA molecules, typically 20-25 base pairs in length, involved in post-transcriptional gene silencing. It can be used in research, but there are also a few therapeutic applications.
In our research, we investigated the inhibition of EGF-R using a predesigned siRNA in F1-4 CHO cancer cells, which express EGF-R with GFP so that it can be detected based on fluorescence.
We transfected F1-4 cells and analyzed the cells with fluorescence microscopy and flow cytometry.
Our experiments were successful, as about 30% of the cells showed low fluorescence compared to the control cells.
The successful knock down means that this siRNA can be used for further experiments in the project in the future.

EGF-R, siRNA, CHO cells, Gene silencing, Cancer therapy, RNAi, Cell signaling