dc.contributor.advisor	Borsos, Antal
dc.contributor.author	Óvári, László
dc.contributor.department	Klinikai orvostudományok doktori iskola	hu
dc.contributor.submitterdep	DE--OEC--Általános Orvostudományi Kar --
dc.date.accessioned	2009-11-05T08:34:09Z
dc.date.available	2009-11-05T08:34:09Z
dc.date.defended	2009-11-13
dc.date.issued	2009
dc.description.abstract	Biochemical markers are reliable indicators of sperm maturity and function. The major hallmark among biochemical markers is the 70 kDa heat shock protein, the HspA2. It proved to be very useful in assessment of sperm immaturity and fertilization potential. Expression of HspA2 is developmentally regulated, having a characteristic dual wave expression pattern. The first peak is in the round spermatocytes, the second a major peak is expressed in the elongating spermatids. Concerning the function of HspA2, it is part of the synaptonemal complex and supports the meiosis in round spermatocytes; abnormal expression therefore may cause meiosis defects, disomies. In the elongation phase HspA2 as chaperone protein facilitates assembly, folding and transport of different proteins among them protamins, DNA repair enzymes and proteins necessary for membrane remodeling. The low expression of HspA2 resulted in different forms of arrested nuclear and cytoplasmic maturities, like persistent histones, DNA chain brakes and surplus cytoplasm. Previous studies on HspA2 theory found correlation between different attributes of cellular immaturity in the semen samples. Other researches studied relationship between meiotic defects and various cellular immaturity forms in semen samples, and there was also strong correlation between them. These studies validate the HspA2 theory, but the intracellular relationship between the HspA2 related defects remained unclear. My concern in this thesis was to find intracellular correlation between different HspA2 related defects such as nuclear and cellular immaturity attributes and meiotic nondisjunction. To test supposed co-occurrence of abnormalities double probing technique of individual spermatozoa has been developed. In the first experiment by consecutive AB and FISH probing we found that arrested nucleoprotein replacement and meiotic non disjunction are related within individual spermatozoa. This study validates our hypothesis on the central role of HspA2 in the spermatogenesis. In another study AB staining was combined with CK immunostaining, ISNT, and caspase-3 immunocytochemistry. Data of this study proved that different attributes of arrested maturity in the nuclear and cellular compartment, persistent histones, surplus cytoplasm and DNA chain bakes are related in the same cell, moreover the degree of abnormalities were also conforming (kappa=0.8). The apoptotic activity was also related to immaturity. These facts support the theory that HspA2 has central role in the spermatogenesis. However in low proportion there is heterogeneity in staining pattern. This is important for two reasons; first it proves that sperm after the defected maturation step may follow its changes in several pathways. Second the arrested spermiogenesis can not be examined correctly just by one biochemical probe only. In the third study detailed morphometrical evaluation of the sperm head and tail was carried out in order to find differences between native, non decondensed spermatozoa with haploid and disomic set. Meanwhile there was significant difference in morphometric parameters such as head area, head area and the short axis between haploid and aneuploid cells by objective morphometry, disomic sperm heads were larger, but the by evaluation of distribution data were inconsistent, there was also a substantial (70%) overlapping in the of the values of these two populations, and disomic sperm heads were among the microcephalic spermatozoa also. Our data proves that the visual assessment in the selection of good chromosomal quality sperm using morphometric aspects is unreliable. -----	hu_HU
dc.format.extent	69 p.	hu_HU
dc.identifier.uri	http://hdl.handle.net/2437/89925
dc.language.iso	hu	hu_HU
dc.language.iso	en	hu_HU
dc.subject	spermatozoa	hu_HU
dc.subject	HspA2	hu_HU
dc.subject.discipline	Klinikai orvostudományok	hu
dc.subject.sciencefield	Orvostudományok	hu
dc.title	Relationship Among Attributes of Sperm Immaturaty, Chromosomal Non Disjunction -Results by Double Probing of Individual Spermatozoon	hu_HU
dc.title.translated	A spermiumok különböző éretlenségi formáinak és kromoszóma non diszjunkcióinak kapcsolata -eredmények ugyanazon sejtek kettős festésével	hu_HU
dc.type	PhD, doktori értekezés	hu

Relationship Among Attributes of Sperm Immaturaty, Chromosomal Non Disjunction -Results by Double Probing of Individual Spermatozoon

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