A critical evaluation of methods used for S-genotyping: from trees to DNA level

A tétel rövid nézete
dc.creatorHalász, J.
dc.creatorHegedűs, A.
dc.date2006-04-19
dc.date.accessioned2021-06-28T09:59:39Z
dc.date.available2021-06-28T09:59:39Z
dc.descriptionFruit setting behaviour of fruit trees remains to be in the focus of plant breeders and growers. Realizing that most species (cherry, apple, pear etc.) are self-incompatible and certain cultivars are cross-incompatible, mutual fertility properties and their reliable determination are of great interest. This review gives a comprehensive description of all known S-genotyping procedures, i.e. the classical fruit set analysis after open field test crosses; pollen tube growth monitoring with fluorescent microscopy; stylar ribonuclease electrophoresis (using different types of isoelectric focusing and 2-dimension polyacrilamide gel electrophoresis); as well as the most recent polymerase chain reaction based DNA-level analyses and DNA sequencing. The review presented not only gives a compilation of the bases of the methods described but also provides a critical evaluation and a comparative characterization of their applicability.
dc.formatapplication/pdf
dc.identifierhttps://ojs.lib.unideb.hu/IJHS/article/view/631
dc.identifier10.31421/IJHS/12/2/631
dc.identifier.urihttp://hdl.handle.net/2437/314368
dc.languageeng
dc.publisherUniversity of Debrecen
dc.relationhttps://ojs.lib.unideb.hu/IJHS/article/view/631/630
dc.rightsCopyright (c) 2018 International Journal of Horticultural Science
dc.sourceInternational Journal of Horticultural Science; Vol. 12 No. 2 (2006); 19-29.
dc.source2676-931X
dc.source1585-0404
dc.source10.31421/IJHS/12/2
dc.subjectF-box
dc.subjectNEpHGE
dc.subjectPCR
dc.subjectRosaceae
dc.subjectself-incompatibility
dc.subjectS-genotype
dc.subjectS-ribonuclease
dc.titleA critical evaluation of methods used for S-genotyping: from trees to DNA level
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
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International Journal of Horticultural Science