A critical evaluation of methods used for S-genotyping: from trees to DNA level

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dc.contributor.authorHalász, J.
dc.contributor.authorHegedűs, A.
dc.date.accessioned2021-06-28T09:59:39Z
dc.date.available2021-06-28T09:59:39Z
dc.date.issued2006-04-19
dc.description.abstractFruit setting behaviour of fruit trees remains to be in the focus of plant breeders and growers. Realizing that most species (cherry, apple, pear etc.) are self-incompatible and certain cultivars are cross-incompatible, mutual fertility properties and their reliable determination are of great interest. This review gives a comprehensive description of all known S-genotyping procedures, i.e. the classical fruit set analysis after open field test crosses; pollen tube growth monitoring with fluorescent microscopy; stylar ribonuclease electrophoresis (using different types of isoelectric focusing and 2-dimension polyacrilamide gel electrophoresis); as well as the most recent polymerase chain reaction based DNA-level analyses and DNA sequencing. The review presented not only gives a compilation of the bases of the methods described but also provides a critical evaluation and a comparative characterization of their applicability.en
dc.formatapplication/pdf
dc.identifier.citationInternational Journal of Horticultural Science, Vol. 12 No. 2 (2006) , 19-29.
dc.identifier.doihttps://doi.org/10.31421/IJHS/12/2/631
dc.identifier.eissn2676-931X
dc.identifier.issn1585-0404
dc.identifier.issue2
dc.identifier.jatitleInt. j. hortic. sci.
dc.identifier.jtitleInternational Journal of Horticultural Science
dc.identifier.urihttps://hdl.handle.net/2437/314368en
dc.identifier.volume12
dc.languageen
dc.relationhttps://ojs.lib.unideb.hu/IJHS/article/view/631
dc.rights.accessOpen Access
dc.rights.ownerInternational Journal of Horticultural Science
dc.subjectF-boxen
dc.subjectNEpHGEen
dc.subjectPCRen
dc.subjectRosaceaeen
dc.subjectself-incompatibilityen
dc.subjectS-genotypeen
dc.subjectS-ribonucleaseen
dc.titleA critical evaluation of methods used for S-genotyping: from trees to DNA levelen
dc.typefolyóiratcikkhu
dc.typearticleen
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International Journal of Horticultural Science (DE-journals)