Comparing levels of heterozygosity and loss-of-heterozygosity regions in probiotic yeasts

A tétel rövid nézete
dc.contributor.advisorPfliegler, Valter Péter
dc.contributor.authorMbeki, Zizipo
dc.contributor.departmentDE--Természettudományi és Technológiai Kar--Biológiai és Ökológiai Intézethu_HU
dc.date.accessioned2021-04-29T20:53:14Z
dc.date.available2021-04-29T20:53:14Z
dc.date.created2021
dc.description.abstractThis study is an analysis and comparison of heterozygosity levels and LOH regions of four probiotic yeast strains. LOH events are primarily caused by stressful conditions like DNA damage in the form of double-strand breaks. Four strains were mapped and sequenced onto the S288c S.cerevisiae reference genome to produce viable indels and SNPs. Galaxy, SAMtools, Picard tools, and GATK were utilised to determine and compare the levels of heterozygosity in the isolates. Visualisation of the results was done using allele plots that were accompanied by YMAP images. A steady decline in heterozygosity levels was observed amongst the strains, which indicates the presence of microevolution events such as mitotic recombination.hu_HU
dc.description.courseBiochemical Engineeringhu_HU
dc.description.degreeBSc/BAhu_HU
dc.format.extent43hu_HU
dc.identifier.urihttp://hdl.handle.net/2437/307853
dc.language.isoenhu_HU
dc.subjectLoss of heterozygosityhu_HU
dc.subjectProbiotic Yeastshu_HU
dc.subjectHeterozygosityhu_HU
dc.subjectSingle Nucleotide Polymorphismshu_HU
dc.subjectGenome analysishu_HU
dc.subjectCopy Number Variantshu_HU
dc.subjectYeast geneticshu_HU
dc.subject.dspaceDEENK Témalista::Biológiai tudományokhu_HU
dc.titleComparing levels of heterozygosity and loss-of-heterozygosity regions in probiotic yeastshu_HU
Fájlok
Gyűjtemények
Theses (Faculty of Science and Technology)