Investigations on the role of microRNAs and gene polymorphisms in the pathogenesis of primary Sjögren’s syndrome

Chen, Ji-Qing

Investigations on the role of microRNAs and gene polymorphisms in the pathogenesis of primary Sjögren’s syndrome

dc.contributor.advisor	Zeher, Margit
dc.contributor.author	Chen, Ji-Qing
dc.contributor.authorvariant	Chen, Jiqing
dc.contributor.department	Petrányi Gyula Klinikai immunológiai és allergológiai doktori iskola	hu
dc.contributor.submitterdep	DE--Általános Orvostudományi Kar -- Klinikai Immunológiai Tanszék
dc.date.accessioned	2017-04-25T09:54:00Z
dc.date.available	2017-04-25T09:54:00Z
dc.date.created	2017	hu_HU
dc.date.defended	2017-05-08
dc.description.abstract	Our study was the first in the literature which focused on the VDR gene polymorphisms in pSS; however, there were no differences in the distribution of BsmI, TaqI, ApaI, and FokI genotypes and the common haplotypes between pSS patients and healthy controls. We hypothesize that these polymorphisms of the VDR gene are not associated with the development of pSS in the Hungarian population. We observed the over-expression of miR-155 in the PBMCs of pSS patients. SOCS1 gene was also over-expressed in patients. This unanticipated phenomenon might be a laboratory characteristic of Sjögren's syndrome, and presumably a consequence of the noteworthy difference in the pSS immune system reacting with EBV. Our results on miRNAs expression profiles indicated that in SLE patients 135, while in pSS patients 26 miRNAs showed altered expression. Interestingly, the 25 miRNAs including miR-146a, miR-16 and miR-21, which were over-expressed in pSS patients, were found to be elevated in SLE group, as well. On the contrary, we observed the down-regulation of miR-150-5p, which is a novel and unique finding in pSS. Levels of several miRNAs over-expressed in SLE, were not changed in pSS, such as miR-148a-3p, miR-152, miR-155, miR-223, miR-224, miR-326 and miR-342. Expression levels of miR-223-5p, miR-150-5p, miR-155-5p and miR-342-3p, which miRNAs are potentially linked to B cell functions, showed associations with the B cell proportions within peripheral blood mononuclear cells. The observed differences in miRNA expression profiles and the better understanding of immune regulatory mechanisms of miRNAs may help to elucidate the pathogenesis of SLE and pSS.	hu_HU
dc.description.corrector	de
dc.format.extent	98	hu_HU
dc.identifier.uri	http://hdl.handle.net/2437/238462
dc.language.iso	en	hu_HU
dc.subject	Primary Sjögren’s syndrome (pSS)	hu_HU
dc.subject	vitamin D receptor (VDR)
dc.subject	gene polymorphisms
dc.subject	microRNA (miRNA)
dc.subject	Suppressor of cytokine signaling (SOCS)
dc.subject.discipline	Klinikai orvostudományok	hu
dc.subject.sciencefield	Orvostudományok	hu
dc.title	Investigations on the role of microRNAs and gene polymorphisms in the pathogenesis of primary Sjögren’s syndrome	hu_HU
dc.title.translated	MikroRNS-ek és genetikai polimorfizmusok szerepének vizsgálata primer Sjögren-szindróma pathogenezisében	hu_HU