Molecular cloning of Anuroctoxin gene into pPICZαA: A yeast expression vector

A tétel rövid nézete
dc.contributor.advisorPanyi, György
dc.contributor.advisorNaseem, Muhammad Umair
dc.contributor.authorGembiczki, Júlia
dc.contributor.departmentDE--Természettudományi és Technológiai Kar--Biológiai és Ökológiai Intézethu_HU
dc.date.accessioned2021-04-22T08:34:04Z
dc.date.available2021-04-22T08:34:04Z
dc.date.created2021-04-21
dc.description.abstractAnuroctoxin gene was amplified from source plasmid and cloned into pPICZαA vector using the restriction ligation approach. The recombinant vector was transformed into bacterial strain E. coli using heat shock method. Finally, the correct cloning of gene into desired vector was authenticated by DNA sequencing. In conclusion, I successfully constructed pPICZαA-AnTx vector for the expression of Anuroctoxin in Pichia pastoris expression system.hu_HU
dc.description.courseBiomérnökhu_HU
dc.description.degreeBSc/BAhu_HU
dc.format.extent23hu_HU
dc.identifier.urihttp://hdl.handle.net/2437/306741
dc.language.isoenhu_HU
dc.subjectmolecularhu_HU
dc.subjectcloninghu_HU
dc.subjecttoxinhu_HU
dc.subjectAntxhu_HU
dc.subjectpcrhu_HU
dc.subject.dspaceDEENK Témalista::Biológiai tudományokhu_HU
dc.titleMolecular cloning of Anuroctoxin gene into pPICZαA: A yeast expression vectorhu_HU
Fájlok
Gyűjtemények
Hallgatói dolgozatok (Biotechnológiai Intézet)