Production of transgenic bean callus via genetic transformation by DNA-coated tungsten particles

A tétel rövid nézete
dc.creatorEissa Ahmed, E.
dc.date2003-10-20
dc.date.accessioned2021-06-28T09:58:39Z
dc.date.available2021-06-28T09:58:39Z
dc.descriptionCallus cultures were induced from hypocotyl of young bean seedlings. The B5 medium completed with 1 mg/1 KIN and 2mg/1 2,4-D proved the best. Callus developed and maintenaned on B5 medium supplemented with 1mg/1 kinetin and 2mg/I 2,4-D. The B5 medium supplemented with 1mg/1 KIN and 2mg/1 2,4-D induced much more callus than half strength MS medium supplemented with 0.5 or 0.75mg/1 BA and 0.1 mg/1 NAA. The results demonstrate that GeneboosterTM is convenient method to obtain transient gene expression in callus of bean. The results have shown that the bean callus shot by GeneboosterTM can be transformed to get (kanamycin-resistant and stress mannitol­tolerant) calli. The presence of mannitol-dehydrogenase gene (mt/) was verified by PCR, showing the integration of mt/ gene carried by two plasmids. Co-transformed calli were selected after bombardment on kanamycin, mannitol and (kanamycin+mannitop-containing media. Data of molecular analysis (PCR) confirmed the insertion of mtl gene in the genome of mannitol-tolerant callus lines.
dc.formatapplication/pdf
dc.identifierhttps://ojs.lib.unideb.hu/IJHS/article/view/404
dc.identifier10.31421/IJHS/9/3-4/404
dc.identifier.urihttp://hdl.handle.net/2437/314210
dc.languageeng
dc.publisherUniversity of Debrecen
dc.relationhttps://ojs.lib.unideb.hu/IJHS/article/view/404/403
dc.rightsCopyright (c) 2018 International Journal of Horticultural Science
dc.sourceInternational Journal of Horticultural Science; Vol. 9 No. 3-4 (2003); 43-47.
dc.source2676-931X
dc.source1585-0404
dc.source10.31421/IJHS/9/3-4
dc.subjectPhaseolus vulgaris
dc.subjectbean
dc.subjectGenebooster
dc.subjecttransformation
dc.subjectstress
dc.subjectmannitol
dc.titleProduction of transgenic bean callus via genetic transformation by DNA-coated tungsten particles
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
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International Journal of Horticultural Science