This study focuses on the isolation and characterization of bacteriophages from wastewater samples to combat multidrug-resistant P. aeruginosa, as well as optimizing phage DNA extraction methods for long-read sequencing. This study focuses on the isolation and characterization of bacteriophages from wastewater samples to combat multidrug-resistant P. aeruginosa, as well as optimizing phage DNA extraction methods for long-read sequencing.