Cell specific expression of HCN2

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dc.contributor.advisorUray, Karen
dc.contributor.advisordeptDebreceni Egyetem::Általános Orvostudományi Kar::Orvosi Vegytani Intézethu_HU
dc.contributor.authorTurarova, Botagoz
dc.contributor.departmentDE--Általános Orvostudományi Karhu_HU
dc.contributor.opponentPóliska, Szilárd
dc.contributor.opponentdeptBiokémiai és Molekuláris Biológiai Intézethu_HU
dc.date.accessioned2022-06-10T15:31:53Z
dc.date.available2022-06-10T15:31:53Z
dc.date.created2022-05-07
dc.description.abstractThe current research was established for the examination of cell specific HCN2 expressional changes in response to stretch in intestinal smooth muscle layer. The expression of HCN2 channel was investigated in macrophages and human intestinal smooth muscle cells (hISMC). Cells were treated with either dimethyl sulphoxide (DMSO) or BMS (inhibitor of I kappa B kinase) to evaluate the response in HCN2 expression when NF-kB pro-inflammatory signalling pathway is blocked. HCN2 protein measurements by Western Blot demonstrated that in macrophages the expression of HCN2 was decreased after ECS stretching, while qRT-PCR showed the significantly decreased mRNA level in hISMC in the ECS protocol. Additonally, BMS treated cells showed higher mRNA signal than in cells treated with control solvent in the hISMC.hu_HU
dc.description.coursemolekuláris biológiahu_HU
dc.description.courselangangolhu_HU
dc.description.coursespecBiokémia-genomikahu_HU
dc.description.degreeMSc/MAhu_HU
dc.format.extent37hu_HU
dc.identifier.urihttp://hdl.handle.net/2437/334425
dc.language.isoenhu_HU
dc.subjectHCN2hu_HU
dc.subjectexpression
dc.subjection channels
dc.subjectsmall intestine
dc.subjectsmooth muscle cells
dc.subjectmacrophages
dc.subjectGI motility
dc.subject.dspaceDEENK Témalista::Biológiai tudományokhu_HU
dc.titleCell specific expression of HCN2hu_HU
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