DHAC-induced transgene overexpression in 35S-gshI GMO poplar (Populus × canescens)
| dc.contributor.author | Gyulai, Gábor | |
| dc.date.accessioned | 2021-06-28T10:59:01Z | |
| dc.date.available | 2021-06-28T10:59:01Z | |
| dc.date.issued | 2007-11-15 | |
| dc.description.abstract | Relative gene expression levels of transgene gshI (γ-glutamylcysteine synthetase cloned from E. coli) were analyzed by qRT-PCR in two transgenic poplar (Populus × canescens) clones (11ggs and 6lgl) and wild type (WT). An extremely high expression level of transgene gshI was observed in the 6lgl clone (13.5-fold) compared to 11ggs (1.0) samples, which level was doubled (1.8-fold) in the DHAC (5.6-dihydro-5'-azacytidine hydrochloride) treated (at 10 -4 M for 7 days) 6lgl samples but not in the 11ggs clone (0.4-fold). Contrary to this result, relative copy number of transgene gshI in the 6lgl clone was found to be less 60% less (1.0) then in the 11ggs samples (1.6). Relative expression levels of proper poplar gene gsh1-poplar showed significantly higher responsiveness to DHAC treatment than transgene gshI with the highest expression level in the untransformed (WT) poplar clone (19.7-fold) compared to transformed 6lgl (8.7-fold) and 11ggs (2.5-fold) clones. For internal controls constitutively expressed housekeeping genes a-tubulin were applied. For data analysis, the 2 −ΔΔCt method was used. DHAC applied in long-term cultures (27 days) at low concentrations (10 -8 – 10 -6 M) showed morphogenetic activity by initiating de novo root development of leaf discs. | en |
| dc.format | application/pdf | |
| dc.identifier.citation | Acta Agraria Debreceniensis, No. 27 (2007) , 78-83 | |
| dc.identifier.issn | 2416-1640 | |
| dc.identifier.issue | 27 | |
| dc.identifier.jatitle | Acta agrar. Debr. | |
| dc.identifier.jtitle | Acta Agraria Debreceniensis | |
| dc.identifier.uri | https://hdl.handle.net/2437/316627 | en |
| dc.language | en | |
| dc.relation | https://ojs.lib.unideb.hu/actaagrar/article/view/3107 | |
| dc.rights.access | Open Access | |
| dc.subject | qRT-PCR | en |
| dc.subject | DHAC | en |
| dc.subject | transgenic poplar | en |
| dc.title | DHAC-induced transgene overexpression in 35S-gshI GMO poplar (Populus × canescens) | en |
| dc.type | folyóiratcikk | hu |
| dc.type | article | en |
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