Inland and foreign Erwinia amylovora isolates by carbohydrate utilization

A tétel rövid nézete
dc.contributor.authorVégh, Anita
dc.contributor.authorHevesi, Mária
dc.contributor.authorPalkovics, László
dc.date.accessioned2021-06-28T10:57:07Z
dc.date.available2021-06-28T10:57:07Z
dc.date.issued2010-11-10
dc.description.abstractFire blight, a plant disease caused by the bacterium Erwinia amylovora, produces serious losses in apple and pear orchards all over the world. Since the appearance of fire blight in Hungary (Hevesi, 1996) Erwinia amylovora isolates were collected in different years, from different hosts and areas in order to establish gene bank for future epidemiological studies. We had isolates from foreign countries as well. The aim of our research was to compare all of the Hungarian and foreign isolates by carbohydrate utilization.  In our experiments effect of carbohydrates on E. amylovora multiplication was determined using API 50 CH strip (bioMérieux, France). By the API 50 CH strip method we checked a number of unstudied carbohydrates. The results of the tests shows colour changes. Based on utilization of 49 carbohydrates of API 50 CH kit by E. amylovora isolates, two groups of carbohydrates can be defined: “Utilized” - and “Not utilized”  carbohydrates. All isolates utilized 20 different carbohydrates after 164 hour incubation. Conversely, isolates also could be divided into four groups (1, 2, 3, 4) by arbutin and raffinose utilization. In group 1.-isolates utilize arbutin; 2.- utilize raffinose; 3.- utilize both arbutin and raffinose; 4. -utilize neither arbutin nor raffinose. Presumably carbohydrate content of nectar could play an important role on invasion of the (E. amylovora) bacterium via flower. It could be concluded that the carbohydrate utilization – completed with genetic analysis – can be used for characterization of Erwinia amylovora isolates.en
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dc.identifier.citationActa Agraria Debreceniensis, No. 39 (2010): Special crop protection issue , 29-33
dc.identifier.doihttps://doi.org/10.34101/actaagrar/39/2734
dc.identifier.issn2416-1640
dc.identifier.issue39
dc.identifier.jatitleActa agrar. Debr.
dc.identifier.jtitleActa Agraria Debreceniensis
dc.identifier.urihttps://hdl.handle.net/2437/316351en
dc.languageen
dc.relationhttps://ojs.lib.unideb.hu/actaagrar/article/view/2734
dc.rights.accessOpen Access
dc.subjectfire blighten
dc.subjectErwinia amylovoraen
dc.subjectAPI 50 CH stripen
dc.subjectcarbohydrate utilizationen
dc.titleInland and foreign Erwinia amylovora isolates by carbohydrate utilizationen
dc.typefolyóiratcikkhu
dc.typearticleen
dc.type.detailedidegen nyelvű folyóiratközlemény hazai lapbanhu
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