The effect of β-glucan, carotenoids, oligosaccharides and anthocyanins on bacteria groups of excreta in broiler chickens

A tétel rövid nézete
dc.creatorCsernus, Brigitta
dc.creatorBiró, Sándor
dc.creatorBabinszky, László
dc.creatorStündl, László
dc.creatorRemenyik, Judit
dc.creatorPesti-Asbóth, Georgina
dc.creatorOláh, János
dc.creatorCzeglédi, Levente
dc.date2022-05-26
dc.date.accessioned2022-05-26T16:58:19Z
dc.date.available2022-05-26T16:58:19Z
dc.descriptionThis study was conducted to examine the effect of natural compounds, such as β-glucan, carotenoids, oligosaccharides, and anthocyanins in the diet on bacteria gropus of excreta in Ross 308 broiler chickens. Chickens were fed 5 diets: control (basal) diet, a diet supplemented by β-glucan at 0.05%, and diets supplemented by carotenoids, oligosaccharides, or anthocyanins at 0.5% of each compound. On experimental day 19, excreta were collected to determine the proportion of Lactobacillus, Bifidobacterium, Campylobacter, Clostridium, and Escherichia coli. Samples were collected aseptically and snap-frozen in liquid nitrogen. Bacterial DNA was isolated from samples, then polymerase chain reaction using primer pairs designed to the 16S rDNA of bacterial groups were applied to define the proportion of the mentioned bacteria. Another universal primer pair was used to amplify a region of 16S rDNA of all the examined bacteria. Proportion of each bacterial groups was determined relatively to the intensity of universal PCR product band by gel documenting system and ImageLab software. Based on the results, carotenoids and anthocyanins increased the proportion of Bifidobacterium, which might imply the beneficial effects of the mentioned compounds on the bacteria composition of excreta.
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dc.identifierhttps://ojs.lib.unideb.hu/actaagrar/article/view/10639
dc.identifier10.34101/actaagrar/1/10639
dc.identifier.urihttp://hdl.handle.net/2437/333721
dc.languageeng
dc.publisherFaculty of Agricultural and Food Sciences and Enviromental Management of the Debrecen University, Debrecen.
dc.relationhttps://ojs.lib.unideb.hu/actaagrar/article/view/10639/9782
dc.rightsCopyright (c) 2022 Acta Agraria Debreceniensis
dc.rightshttps://creativecommons.org/licenses/by/4.0
dc.sourceActa Agraria Debreceniensis; No. 1 (2022); 15-20
dc.source2416-1640
dc.source1587-1282
dc.subjectnatural compounds
dc.subjectchicken
dc.subjectmicrobiota
dc.subjectPCR
dc.titleThe effect of β-glucan, carotenoids, oligosaccharides and anthocyanins on bacteria groups of excreta in broiler chickens
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typePeer-reviewed Article
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Acta Agraria Debreceniensis