The effect of β-glucan, carotenoids, oligosaccharides and anthocyanins on bacteria groups of excreta in broiler chickens

A tétel rövid nézete
dc.contributor.authorCsernus, Brigitta
dc.contributor.authorBiró, Sándor
dc.contributor.authorBabinszky, László
dc.contributor.authorStündl, László
dc.contributor.authorRemenyik, Judit
dc.contributor.authorPesti-Asbóth, Georgina
dc.contributor.authorOláh, János
dc.contributor.authorCzeglédi, Levente
dc.date.accessioned2022-05-26T16:58:19Z
dc.date.available2022-05-26T16:58:19Z
dc.date.issued2022-05-26
dc.description.abstractThis study was conducted to examine the effect of natural compounds, such as β-glucan, carotenoids, oligosaccharides, and anthocyanins in the diet on bacteria gropus of excreta in Ross 308 broiler chickens. Chickens were fed 5 diets: control (basal) diet, a diet supplemented by β-glucan at 0.05%, and diets supplemented by carotenoids, oligosaccharides, or anthocyanins at 0.5% of each compound. On experimental day 19, excreta were collected to determine the proportion of Lactobacillus, Bifidobacterium, Campylobacter, Clostridium, and Escherichia coli. Samples were collected aseptically and snap-frozen in liquid nitrogen. Bacterial DNA was isolated from samples, then polymerase chain reaction using primer pairs designed to the 16S rDNA of bacterial groups were applied to define the proportion of the mentioned bacteria. Another universal primer pair was used to amplify a region of 16S rDNA of all the examined bacteria. Proportion of each bacterial groups was determined relatively to the intensity of universal PCR product band by gel documenting system and ImageLab software. Based on the results, carotenoids and anthocyanins increased the proportion of Bifidobacterium, which might imply the beneficial effects of the mentioned compounds on the bacteria composition of excreta.en
dc.formatapplication/pdf
dc.identifier.citationActa Agraria Debreceniensis, No. 1 (2022) , 15-20
dc.identifier.doihttps://doi.org/10.34101/actaagrar/1/10639
dc.identifier.issn2416-1640
dc.identifier.issue1
dc.identifier.jatitleActa agrar. Debr.
dc.identifier.jtitleActa Agraria Debreceniensis
dc.identifier.urihttps://hdl.handle.net/2437/333721en
dc.languageen
dc.relationhttps://ojs.lib.unideb.hu/actaagrar/article/view/10639
dc.rights.accessOpen Access
dc.rights.ownerby the Author(s)
dc.subjectnatural compoundsen
dc.subjectchickenen
dc.subjectmicrobiotaen
dc.subjectPCRen
dc.titleThe effect of β-glucan, carotenoids, oligosaccharides and anthocyanins on bacteria groups of excreta in broiler chickensen
dc.typefolyóiratcikkhu
dc.typearticleen
dc.type.detailedidegen nyelvű folyóiratközlemény hazai lapbanhu
Fájlok
Eredeti köteg (ORIGINAL bundle)
Megjelenítve 1 - 1 (Összesen 1)
Nincs kép
Név:
pdf
Méret:
577.09 KB
Formátum:
Adobe Portable Document Format
Letöltés
Gyűjtemények
Acta Agraria Debreceniensis (DE-journals)