Doxorubicin is a strong antineoplastic agent which can affects intensely on cells during the treatment. Metformin were shown to possess cardioprotective effect. This cardiactoxicity can be reduced after we treat these cells with Metformin. The cells were survived more under the DOX treatment with the help of Metformin. The aim of this study is to determine the extent to which MET can prevent the autophagy of DOX-induced cells toxicity. In short, result of this work may figure out the usefulness of Metformin and further improve the quality for pathological situations which use Doxorubicin in treatment.