Theses (Faculty of Pharmacy)
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Tétel Korlátozottan hozzáférhető Exploring PD-L1 and PD-L2 as Possible Biomarkers in Renal CancerSEYED PIRAN, SEYED BEHRAD; Szabó , Zsuzsanna; Gyógyszerésztudományi Kar; DE--Gyógyszerésztudományi Kar; Dobos, Nikoletta; Szabó, Erzsébet; Gyógyszerésztudományi KarThis study explores how two proteins, PD-L1 and PD-L2, may help predict treatment results in people with kidney cancer, specifically renal cell carcinoma (RCC). Researchers compared cancer tissue and nearby healthy kidney tissue from 20 patients and found both proteins were expressed more in tumors. Especially in aggressive tumors (Grade 4), PD-L1 and PD-L2 levels were much higher, suggesting a link between these proteins and tumor severity. One case showed unusually high PD-L1 even in a less aggressive tumor, hinting at differences between individual tumors. The findings suggest that targeting both proteins could improve treatment results, especially for high-risk patients. The study supports the use of these proteins to better match patients with the right immune therapy.Tétel Korlátozottan hozzáférhető Synthetic Derivatives of C. sativa as Potential Drug Candidates Against Cutaneous Melanoma CellsGholami, Soheyla; Szabó , Erzsébet; Gyógyszerésztudományi Kar; DE--Gyógyszerésztudományi Kar; Tosaki, Agnes; Szabó, Zsuzsanna; Gyógyszerésztudományi KarThis study looks at two synthetic compounds made from Cannabis sativa, named PFD-11A and PFD-35/II, and how they affect skin cancer cells called melanoma. These cancer cells are usually hard to treat, so new drug options are needed. The researchers tested the compounds on two different melanoma cell lines and found that PFD-35/II was more effective at killing the cells. The drugs reduced the cancer cells’ ability to grow and survive over time. They also seemed to work by triggering processes like autophagy and cell death. The findings suggest these compounds could be useful for treating melanoma, but more research, especially in animals, is needed to confirm this.Tétel Korlátozottan hozzáférhető Development of Ketoprofen Orally Disintegrating Tablets (ODT) for Pediatric UseMohammadian, Kimia; Vasvári, Gábor; Gyógyszerésztudományi Kar; DE--Gyógyszerésztudományi Kar; Rusznyák, Ágnes; Nemes, DánielThis thesis presents the development of an orally disintegrating tablet (ODT) formulation containing ketoprofen, intended for pediatric use. The aim was to create a child-friendly dosage form with rapid disintegration, adequate mechanical strength, and acceptable taste. A range of excipients, including co-processed blends of lactose, microcrystalline cellulose (MCC), and low-substituted hydroxypropyl cellulose (L-HPC), were evaluated to optimize the formulation. Comprehensive assessments were conducted on hardness, disintegration time, and dissolution behavior to identify the most effective composition. The findings demonstrate the potential of this formulation approach to enhance pediatric compliance and therapeutic effectiveness. The study contributes to the advancement of innovative drug delivery systems tailored for pediatric patients.Tétel Korlátozottan hozzáférhető Reported cutaneous allergies to antimicrobials in EudraVigilanceChiwara, Tonderai Asher; László , Horváth; Gyógyszerésztudományi Kar::Gyógyszerfelügyelet és Gyógyszergazdálkodási Tanszék; DE--Gyógyszerésztudományi KarThe thesis is a retrospective analysis of cutaneous adverse drug reactions that occurred after taking antibiotics, with a study period that covers a 10-year period from 2013 to 2023. The database used is EudraVigilance, and the API's analysed were from the J01 Category of the ATC code system. Keywords used were obtained from MedDRA to determine which Adverse Events were related to drug hypersensitivity. Microsoft Excel was then used to sort the data and provide it in the form of descriptive statistics. The aim was to provide real-time up to date big data on the adverse drug reactions that occurred, which will help healthcare professionals in decision making when prescribing medication.Tétel Korlátozottan hozzáférhető prevalence and patterns of NSAIDs use among university studentsYaqub Ojali, Faeza; Tóth, Bèla; Gyógyszerésztudományi Kar; DE--Gyógyszerésztudományi Kar; Vercsernyés, Miklós Imre; Váradi, Judit; Gyógyszerésztudományi Kar; Gyógyszerésztudományi KarThe thesis explores the prevalence and patterns of NSAIDs use among university students. It highlights how common self-medication is within this population and draws attention to the limited awareness of its potential side effects. The study shows that many students rely on over-the-counter medications without consulting healthcare professionals. Factors influencing their choices include accessibility, perceived safety and lack of knowledge. The findings emphasises the need for targeted educational interventions to promote safer drug use.Tétel Korlátozottan hozzáférhető Formulation of Alginate Nanoparticles from Green Pea ExtractNadri, Ehsan; Bácskay, IIdikó; Sinka, Dávid zsolt; DE--Gyógyszerésztudományi KarThis thesis explores a novel way to harness the health benefits of green pea extract / by improving its stability and bioavailability using sodium alginate-based nanoparticles. Green peas are rich in fiber, antioxidants, and plant-based nutrients with proven benefits for digestion, blood sugar control, heart health, and more. However, their natural compounds are sensitive and degrade easily, limiting their use in medicines or health supplements. To address this, the study developed and tested a nanoparticle encapsulation system using calcium-alginate beads. This method helps protect the green pea extract and enables a controlled, slow release over time—ideal for both pharmaceutical and food applications. Key Findings: • Nanoparticles averaged ~149 nm in size with a stable surface charge (zeta potential: -15.56 mV). • Encapsulation efficiency was high at 92%, meaning most of the extract was successfully enclosed within the beads. • The beads absorbed water well (EWU: ~79%), indicating good swelling behavior. • Dissolution studies showed gradual release, with over 58% of the extract released in one hour. Overall, the research shows that alginate nanoparticles are a promising vehicle for delivering plant-based bioactives like green pea extract in a more effective and stable way.Tétel Korlátozottan hozzáférhető Investigation of fluorinated CBD derivatives protective effects against Doxorubicin cardiotoxicityNgo, Dang Minh Anh; István, Lekli; Kajtár, Richárd; Gyógyszerésztudományi Kar::Gyógyszerhatástani Tanszék; DE--Gyógyszerésztudományi Kar; Király, Jószef; Gyöngyösi, Alexandra; Gyógyszerésztudományi Kar::Biofarmácia Tanszék; Gyógyszerésztudományi Kar::Gyógyszerhatástani TanszékThe thesis aims to investigate the newly synthesized, fluorinated CBD derivatives on their protective effect against cardiotoxicity induced by Doxorubicin, a well-known chemotherapeutic agent. Several tests were carried out, such as antioxidant assays, MTT assay, MitoSOX staining and Western blot analysis. Antioxidant capacity of the derivatives were thoroughly studied, since the elevation of oxidative stress is one of the major mechanisms of Doxorubicin. The results suggested that the flourinated derivatives, especially molecule 17, showed a higher cell viability, better antioxidant effect and thus better cardioprotection, comparing to CBD. However, further research is required to ensure the safety and effect of the compounds.Tétel Korlátozottan hozzáférhető Comparative study of niacinamide cream and gelfamilbakhtiari , helia; Boros-Kósa, Dora; DE--Gyógyszerésztudományi KarThis thesis explores the use of niacinamide, a form of vitamin B3, in skincare. Niacinamide is known for its benefits in improving skin texture, reducing inflammation, and supporting the skin barrier. Two different topical products containing niacinamide cream and gel were prepared and compared. The goal was to see how each one affects the skin and how well niacinamide can be absorbed. Both products were found to be safe and effective, but the gel allowed better delivery of niacinamide into the skin. These results support the use of niacinamide in modern cosmetic and dermatological formulations.Tétel Korlátozottan hozzáférhető pharmaceutical development of vaccineSamimi, Mana; Ujhelyi, Zoltan; DE--Gyógyszerésztudományi KarThis thesis presents a comprehensive study on the pharmaceutical development of vaccines, focusing on scientific, technical, and regulatory processes. It covers key aspects such as antigen selection, adjuvant formulation, clinical trials, manufacturing, and global distribution. Special emphasis is placed on innovative technologies like mRNA platforms and the challenges of vaccine accessibility and hesitancy. The research aims to highlight both current advancements and future directions in vaccinology, contributing to improved public health strategies worldwide.Tétel Korlátozottan hozzáférhető Treatments of acute heart failureNadri, Mehrnaz; Lekli, István; DE--Gyógyszerésztudományi KarAcute Heart Failure (AHF) is a critical cardiovascular condition that leads to a sudden decline in cardiac function, often requiring urgent medical intervention. Unlike chronic heart failure, AHF develops rapidly and is triggered by underlying conditions such as myocardial infarction, hypertension, arrhythmias, or valvular disease. The high morbidity and mortality associated with AHF highlight the importance of timely diagnosis and effective treatment strategies. AHF arises due to various precipitating factors, including myocardial infarction, hypertension, arrhythmias, valvular disease, pulmonary embolism, sepsis, and non-compliance with medications. Myocardial infarction (heart attack) results in ischemic injury, impairing contractility and reducing cardiac output. Hypertension causes chronic pressure overload, leading to left ventricular hypertrophy and heart failure. Arrhythmias, such as atrial fibrillation, disrupt efficient cardiac output, while valvular diseases, including stenosis and regurgitation, increase cardiac workload. Pulmonary embolism obstructs blood flow to the lungs, straining the right heart, whereas sepsis induces systemic inflammation and myocardial dysfunction. Additionally, non-compliance with heart failure medications can precipitate acute decompensation. The pathophysiology of AHF involves structural and functional changes in the heart. Reduced stroke volume results from weakened myocardial contractility, leading to inadequate cardiac output. Increased filling pressure occurs due to blood accumulation in the heart chambers, causing congestion and pulmonary edema. Elevated afterload, characterized by higher vascular resistance, further impairs the heart’s ability to pump efficiently. These disruptions in the cardiac cycle contribute to the worsening of symptoms and disease progression. The primary goal of AHF treatment is to alleviate symptoms, optimize cardiac function, and prevent deterioration. Pharmacological interventions are the cornerstone of management, with various drug classes playing crucial roles. Diuretics, such as furosemide and bumetanide, reduce fluid overload by increasing urine output, alleviating pulmonary and systemic congestion. However, they may cause hypokalemia, hypotension, and renal dysfunction. Vasodilators, including nitroglycerin and nitroprusside, decrease preload and afterload to improve cardiac efficiency and relieve congestion, but they carry risks of hypotension and reflex tachycardia. Inotropic agents, such as dobutamine, milrinone, and levosimendan, enhance myocardial contractility in severe AHF cases, improving cardiac output. However, they can increase the risk of arrhythmias, hypotension, and myocardial oxygen demand. Angiotensin-converting enzyme (ACE) inhibitors and angiotensin II receptor blockers (ARBs) help reduce afterload and prevent cardiac remodeling, improving long-term survival. Despite their benefits, these drugs can cause hyperkalemia, hypotension, and a persistent cough (in the case of ACE inhibitors). Aldosterone antagonists, including spironolactone and eplerenone, counteract fluid retention and cardiac fibrosis, further improving patient outcomes. However, they pose a risk of hyperkalemia and, in the case of spironolactone, gynecomastia. Beta-blockers, such as carvedilol (also α1-blocker), metoprolol, and bisoprolol, reduce sympathetic overactivity, decreasing heart rate and myocardial oxygen demand. These agents prevent cardiac remodeling and reduce hospitalizations but may cause bradycardia, fatigue, and hypotension. Vasopressors, including norepinephrine and dopamine, are essential in cardiogenic shock, maintaining blood pressure and organ perfusion. However, their vasoconstrictive effects can lead to arrhythmias, ischemia, and renal hypoperfusion. Potential drug interactions must be carefully managed in AHF treatment. Loop diuretics combined with NSAIDs can reduce diuretic efficacy and worsen fluid retention. ACE inhibitors or ARBs, when used with potassium-sparing diuretics, significantly increase the risk of hyperkalemia. Beta-blockers combined with calcium channel blockers can cause severe bradycardia, while aldosterone antagonists used alongside NSAIDs can increase the likelihood of renal dysfunction and hyperkalemia. These interactions highlight the importance of individualized therapy and close patient monitoring.Tétel Korlátozottan hozzáférhető Quercetin unveiled: Targeting NF-κB and p53 pathways to combat Uveal MelanomaSunil, Sona; Zsebik , Barbara; Gyógyszerésztudományi Kar::Biofarmácia Tanszék; DE--Gyógyszerésztudományi Kar; Hegedűs , Éva; Fenyvesi, Ferenc; Általános Orvostudományi Kar::Biofizikai és Sejtbiológiai Intézet; Gyógyszerésztudományi Kar::Gyógyszertechnológiai TanszékUveal melanoma is the most frequently diagnosed primary intraocular tumour in adults, with metastasis occurring in nearly 50% of adults, primarily to the liver and the limited treatment options due to its chemoresistance. NF-κB and p53 are key transcription factors regulating cell survival, growth, and apoptosis. Quercetin (QUE), a naturally occurring flavonol, exhibits anti-cancer properties by activating p53 and inhibiting NF-κB signalling through suppression of IκB kinases, therefore preventing translocation to the nucleus. In this study, QUE significantly reduced uveal melanoma cell viability in a concentration-dependent manner (1–100 µM), as demonstrated by MTT assay and Bürker chamber analysis. Fluorescence imaging revealed a decrease in nuclear NF-κB intensity with a corresponding increase in the cytoplasm, while nuclear p53 intensity increased with QUE treatment (10 µM) compared to untreated, indicating altered subcellular localisation of both transcription factors. These results support the potential of QUE as a well-tolerated and effective adjunct in combination therapy for uveal melanoma.Tétel Korlátozottan hozzáférhető Effect of Syndecan-4 knockdown on mitochondrial function in skeletal musclesOgao, Everlyn; Telek-Haberberger, Andrea; Szentesi, Péter; Általános Orvostudományi Kar; DE--Általános Orvostudományi Kar; Papp, Ferenc; Gaál , Botond; Általános Orvostudományi KarSyndecan-4 is a transmembrane proteoglycan that regulates actin cytoskeleton, cell adhesion, and cell migration. Since actin is involved in muscle contraction, we have already investigated skeletal muscle function in syndecan-4 knockdown (SDC4) mice. Skeletal muscle performance was indeed reduced in SDC4 mice. Consequently, it is plausible to assume that mitochondrial functions are altered in SDC4 mice as well. Therefore, we investigated mitochondrial architecture and the key members of mitochondrial dynamics and regulator of mitochondrial calcium uptake MICU1 in syndecan-4 knockdown mice.Tétel Korlátozottan hozzáférhető Formulation and Investigation of CK2 Inhibitor-Loaded Microparticles.IBRAHIM, RAMATU; Fehér , Pálma; Papp, Boglárka; Gyógyszerésztudományi Kar; DE--Gyógyszerésztudományi Kar; Dobos , Nikoletta; Sinka, David; Gyógyszerésztudományi Kar; Gyógyszerésztudományi KarThis thesis presents a well-structured and insightful investigation into the formulation of CK2 inhibitor-loaded microparticles using sodium alginate microbeads. It successfully demonstrates how encapsulating the drug enhances its bioavailability, stability, antioxidant activity, and cytotoxic effect against cancer cells, especially with the aid of Transcutol HP as a solubilizer. The methodology is clear and thorough, with detailed descriptions of formulation, testing, and analysis techniques such as swelling behavior, encapsulation efficiency, dissolution assays, and MTT cytotoxicity tests. Results show that microbeads containing Transcutol HP outperformed other formulations in drug release and antioxidant capacity. The use of the Caco-2 cell line adds validity to the in vitro findings, and the discussion effectively ties together the therapeutic potential of CK2 inhibitors with the advantages of controlled drug delivery systems. Overall, the thesis makes a valuable contribution to pharmaceutical technology and targeted cancer therapy research.Tétel Korlátozottan hozzáférhető Formulation and evaluation of Philadelphus Coronarius containing topical preprationEmadzadeh, Sahand; Pető, Ágota; Gyógyszerésztudományi Kar::Gyógyszertechnológiai Tanszék; DE--Gyógyszerésztudományi Kar; Boros kosa, Dora; Mihaly, Herczeg; Gyógyszerésztudományi Kar::Gyógyszertechnológiai Tanszék; Gyógyszerésztudományi Kar::Gyógyszerészi Kémiai TanszékThe growing interest in plant-based therapies has renewed focus on the use of medicinal plants like Philadelphus coronarius (mock orange) in pharmaceutical formulations. This plant, known for its antimicrobial, anti-inflammatory, and antioxidant properties, was studied for use in topical treatments for skin conditions. Extracts rich in flavonoids and tannins were obtained using ethanol and incorporated into creams, gels, and ointments. The formulations were evaluated for physicochemical properties such as pH, viscosity, spreadability, and consistency, along with stability under various environmental conditions. Microbiological and in vitro tests confirmed the antimicrobial and anti-inflammatory efficacy of the formulations. Among them, the cream formulation demonstrated the best stability, drug release, and therapeutic effectiveness. Overall, the study supports the potential of Philadelphus coronarius as a natural ingredient in dermatological products, though further clinical research is needed.Tétel Korlátozottan hozzáférhető Formulation and Investigation of BGP-15 Containing Gelsharafi, shahab; Pető, Ágota; Gyógyszerésztudományi Kar; DE--Gyógyszerésztudományi Kar; Szúcs , Zsolt; Haimhoffer, ÁdámMy thesis focuses on formulation and investigation of BGP-15 Containing topical gel.BGP-15 ,a compound known for its cytoprotective and anti-inflammatory properties. The primary objective was to create a stable,effectIve gel formulation using gelling agent such as HPMC.Extensive physicochemical characterisation was performed, including pH measurement , texture analysis and in vitro drug release studies. The results demonstrated that the optimized gel formulation provided desirable properties for topical application and maintained the stability and release of BGP-15 effectively. Overall,the study supports the potential use of BGP-15 in dermatological therapies through gel-based delivery systems.Tétel Korlátozottan hozzáférhető Formulation and Evaluation of Sodium Alginate-Based HydrogelsIbrahim, Asma'u; Boros-Kósa, Dóra; Gyógyszerésztudományi Kar::Gyógyszertechnológiai Tanszék; DE--Gyógyszerésztudományi Kar; Bakai-Bereczki, Ilona; Haimhoffer, Ádám; Gyógyszerésztudományi Kar::Gyógyszerészi Kémiai Tanszék; Gyógyszerésztudományi Kar::Gyógyszertechnológiai TanszékThis thesis explores the formulation and evaluation of sodium alginate-based hydrogels as potential wound healing agents. Hydrogels were prepared using sodium alginate crosslinked with calcium chloride and enhanced with nicotinamide, known for its anti-inflammatory and regenerative properties. Key parameters such as pH, texture, drug diffusion, and cytotoxicity were analyzed to assess their suitability for topical application. Results showed that increasing sodium alginate concentration improved gel strength and initial drug release, while maintaining pH within the skin-compatible range. The MTT assay confirmed good biocompatibility at lower alginate concentrations. These findings support the potential of alginate-based hydrogels in modern wound care.Tétel Korlátozottan hozzáférhető Antitumor Effects of Cannabinoid Derivatives on Breast Cancer Cell LinesChukwudi , Ojelubechukwu; Dobos, Nikoletta; Gyógyszerésztudományi Kar; DE--Gyógyszerésztudományi Kar; Lekli, Istvan; Gyógyszerésztudományi KarThe thesis "Antitumor Effects of Cannabinoid Derivatives on Breast Cancer Cell Lines" written by Chukwudi Ojelubechukwu Chioma investigates the antitumor effects of newly synthesized cannabinoid derivatives on breast cancer cell lines. The experimental work focuses on two types of cell lines: MDA-MB-231 (triple-negative breast cancer cell line) and MCF-7 (hormone-receptor positive breast cancer cell line), assessing cytotoxicity of twelve CBD (Cannabidiol) and CBG (Cannabigerol) derivatives. These compounds were synthesized via a Mannich-type reaction and tested using the MTT cell viability assay at different concentrations and time points. Results revealed that CBD derivatives like PFD43I and PFD10/AI were particularly effective against MDA-MB-231, while PFD45I (CBG) and PFD11/A (CBD) had strong effects in reducing the MCF-7 cell viability. The six most potent compounds underwent further IC50 testing, which showed that CBG derivatives had the best effect overall. The findings support further investigation into these compounds as potential breast cancer therapies, with future research aimed at a deeper exploration into their molecular mechanisms.Tétel Korlátozottan hozzáférhető Biological Effects of Newly Synthesized CBD and CBG Derivatives on Colon Cancer Cell LinesUdeagbala, Amarachi Doris; Eva , Sipos; Gyógyszerésztudományi Kar::Gyógyszerhatástani Tanszék; DE--Gyógyszerésztudományi Kar; Arany, Petra; Boros-Olah, Beata Kissne; Gyógyszerésztudományi Kar::Gyógyszertechnológiai Tanszék; Gyógyszerésztudományi KarThe aim of the theses is to examine the biological effects of newly synthesized cannabinoids derivatives on colon cancer cell lines. Cannabidiol is a nonpsychoactive phytocannabinoid that can be found in Cannabis sativa and it possesses numerous pharmacological effects. The therapeutic potential of the CBD and CBG have been investigated in numerous clinical trials for the treatment of various types of cancer. Here the ILKA675 and ILKA676 which are the newly synthesized derivatives of CBD and CBG are examined to assess the cytotoxic and antioxidant effect on HCT116 colon cancer cell lines. The biological assay used in the theses are MTT ((3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)) assay and FRAP (Ferric Reducing Antioxidant Power) assay.Tétel Korlátozottan hozzáférhető Anticancer properties of 12 newly synthesized CBD- and CBG-derivatives against OCM-1 and OCM-3 human uveal melanoma cell linesBui, Linh Dan; Dobos , Nikoletta; Gyógyszerésztudományi Kar::Biofarmácia Tanszék; DE--Gyógyszerésztudományi Kar; Józsa , Liza; Szőke , Kitti; Gyógyszerésztudományi Kar::Gyógyszertechnológiai Tanszék; Gyógyszerésztudományi Kar::Gyógyszerhatástani TanszékUveal melanoma (UM) is the most common eye cancer in adults, with a poor prognosis due to limited effective therapies. Cannabinoids, such as cannabidiol (CBD), have demonstrated antitumor effects in various cancers, making them potential candidates for the treatment of UM. This research investigated the anticancer potential of 12 newly synthesized CBD, cannabigerol (CBG), and their derivatives on OCM-1 and OCM-3 human uveal melanoma cell lines. Determination of IC50 values using MTT cell viability assays identified six potent derivatives, with CBD being the most effective at killing cancer cells. Modified CBG derivatives also exhibited improved antiproliferative effects compared to unmodified CBG. These findings suggest cannabinoids, especially CBD, as promising treatments for UM and provide a foundation for future research into their molecular mechanisms.Tétel Korlátozottan hozzáférhető Pharmacology and Therapeutic Potential of N,N-Dimethyltryptamine: Ischemia-Related ApplicationAghapour Olilo, Benyamin; Gyöngyösi , Alexandra; Gyógyszerésztudományi Kar::Gyógyszerhatástani Tanszék; DE--Gyógyszerésztudományi Kar; Bak , István; Vasvári , GáborThis thesis explores the therapeutic potential of N,N-Dimethyltryptamine (DMT), a naturally occurring psychedelic compound known for its intense but short-lasting psychoactive effects. Recent preclinical and clinical studies suggest that DMT may have neuroprotective, anti-inflammatory, and psychological healing properties, particularly in conditions like treatment-resistant depression, anxiety, and PTSD. Unlike other psychedelics, DMT also exhibits rapid onset and short duration, making it a promising candidate for controlled clinical use with minimal time burden. The thesis also examines DMT’s interactions with sigma-1 receptors, which play a key role in cellular stress response and neuroregeneration. Ethical, regulatory, and pharmacological considerations are discussed to evaluate its future integration into modern psychiatric and neurological therapy. Overall, DMT emerges as a high-potential molecule that warrants further clinical investigation for its role in next-generation therapeutics.