IRF4 Negatively Regulates The Myeloid Blood Cell Development From Pluripotent Stem Cells

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dc.contributor.advisorSzatmári, István
dc.contributor.advisordeptÁltalános Orvostudományi Kar::Biokémiai és Molekuláris Biológiai Intézet
dc.contributor.authorMalik, Abdul
dc.contributor.departmentDE--Általános Orvostudományi Kar
dc.date.accessioned2024-06-04T07:44:37Z
dc.date.available2024-06-04T07:44:37Z
dc.date.created2024-05
dc.description.abstractThe thesis explores the impact of the transcription factor IRF4 on the differentiation of mouse embryonic stem cells (ESCs) into myeloid dendritic cell (DC) progenitors. For this study, I used two chemically inducible ESC clones carrying the IRF4 transgene. This investigation explores the role of IRF4 in regulating this developmental process. Through flow cytometry analysis and gene expression profiling, it was observed that enforced expression of IRF4 led to a decreased percentage of CD45+ and CD34+ cells, indicative of suppression in ESC-derived myeloid blood cell development. Additionally, the results from RT-PCR analyzes revealed an increased expression of IRF4 when the cells were induced by doxycycline both at their undifferentiated stage and during the differentiation process. Together these results suggest that IRF4 suppresses ESC-derived myeloid blood cell development and exerts a general inhibitory effect on ES-DC development.
dc.description.coursemolekuláris biológia
dc.description.courselangangol
dc.description.coursespecBiokémia-genomika
dc.description.degreeMSc/MA
dc.format.extent42
dc.identifier.urihttps://hdl.handle.net/2437/370861
dc.language.isoen
dc.rights.accessHozzáférhető a 2022 decemberi felsőoktatási törvénymódosítás értelmében.
dc.subjectDendritic cells (DCs)
dc.subjectIRF4 (Interferon Regulatory Factor 4)
dc.subjectEmbryonic stem cells (ESC)
dc.subjectDifferentiation
dc.subject.dspaceBiology::Molecular Biology
dc.titleIRF4 Negatively Regulates The Myeloid Blood Cell Development From Pluripotent Stem Cells
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