Effect of selenium supplement on proteome of chicken liver

dc.creatorGulyás, Gabriella
dc.creatorCzeglédi, Levente
dc.creatorProkisch, József
dc.creatorJávor, András
dc.date2012-12-16
dc.date.accessioned2021-06-28T10:56:16Z
dc.date.available2021-06-28T10:56:16Z
dc.descriptionThe aims of the present study were to optimize a two dimensional polyacrylamide gel electrophoresis method to chicken liver proteome and to determine the changes of protein expression caused by selenium. Twelve broiler chicken were used in this experiment. The selenium intake was 0,2 mg/kg in the control group (6 chicken) and 4,25 mg/kg in the experimental group (6 chicken). Using the optimized proteomic approach, we have succesfully separated 747 proteins in the experimental group and 741 proteins in the control group. We found six proteins, wich expressed only in the samples of experimental group. Further investigations need to determine these six proteins with mass spectrometry (MS) and look for the correlation between the physiological effects of selenium and the expression of these proteins.
dc.formatapplication/pdf
dc.identifierhttps://ojs.lib.unideb.hu/actaagrar/article/view/2557
dc.identifier10.34101/actaagrar/50/2557
dc.identifier.urihttp://hdl.handle.net/2437/316200
dc.languageeng
dc.publisherFaculty of Agricultural and Food Sciences and Enviromental Management of the Debrecen University, Debrecen.
dc.relationhttps://ojs.lib.unideb.hu/actaagrar/article/view/2557/2573
dc.sourceActa Agraria Debreceniensis; No. 50 (2012); 9-13
dc.source2416-1640
dc.source1587-1282
dc.subjectchicken liver
dc.subjectproteomics
dc.subjectselenium
dc.subject2D-PAGE
dc.titleEffect of selenium supplement on proteome of chicken liver
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typePeer-reviewed Article
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