Study of alternative oxidase as possible molecular marker for phylogenetic analysis of the Botrytis cinerea

Fájlok
Dátum
Szerzők
Szojka, Anikó
Sándor, Erzsébet
Folyóirat címe
Folyóirat ISSN
Kötet címe (évfolyam száma)
Kiadó
Faculty of Agricultural and Food Sciences and Enviromental Management of the Debrecen University, Debrecen.
Absztrakt
Leírás
Botrytis cinerea (teleomorph Botryotinia fuckeliana (de Bary) Whetzel) is able to attack several economically important plants causing gray rot. Botrytis cinerea species complex includes two cryptic species (B. cinerea and B. pseudocinerea) that tolerate fungicides differently. On the basis of classical taxonomic markers, the two related species are very difficult to be distinguished; therefore, their separation is usually performed using molecular methods based on the time-consuming molecular analysis of several markers. Our goal was to find markers, which are suitable for the differentiation. Testing the nucleotide sequences of the alternative oxidase encoding gene, B. cinerea and B. pseudocinerea strains were clearly differentiated. Moreover, the analysis of the protein sequences of the enzyme with the maximum likelihood method reflected well the taxonomic relationships of the different fungi.
Kulcsszavak
Botrytis cinerea, Botrytis pseudocinerea, alternative oxidase
Forrás