Optimizing surface sampling for healthcare pathogen detection: The effect of sample size and swabbing techniques on pathogen recovery

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dc.contributor.advisorFreytag, Csongor
dc.contributor.advisorCseri, Karolina
dc.contributor.advisordeptMetagenomikai Intézet
dc.contributor.authorAbboud, Eric
dc.contributor.departmentDE--Általános Orvostudományi Kar
dc.contributor.opponentSzatmári, István
dc.contributor.opponentdeptÁltalános Orvostudományi Kar::Biokémiai és Molekuláris Biológiai Intézet
dc.date.accessioned2025-06-06T09:44:44Z
dc.date.available2025-06-06T09:44:44Z
dc.date.created2025
dc.description.abstractThis study evaluates how sampling size and swabbing techniques influence the recovery of pathogens from environmental surfaces in healthcare settings. Stainless steel surfaces were inoculated with bacterial strains, and sampling was done using both individual and pooled methods across 10x10, 5x5, and 2x2 cm areas. Results showed that larger surfaces and pooled sampling increased recovery efficiency. Metagenomic analysis using Oxford Nanopore sequencing enabled rapid and high-resolution identification of bacterial species and antimicrobial resistance markers. Findings support integrating culture-based and sequencing approaches to improve infection control protocols. The study highlights the need for standardized surface sampling strategies in healthcare environments.
dc.description.coursemolekuláris biológia
dc.description.courselangangol
dc.description.coursespecBiokémia-genomika
dc.description.degreeMSc/MA
dc.format.extent35
dc.identifier.urihttps://hdl.handle.net/2437/390988
dc.language.isoen
dc.rights.infoHozzáférhető a 2022 decemberi felsőoktatási törvénymódosítás értelmében.
dc.subjectPathogen recovery
dc.subjectEnvironmental surface sampling
dc.subjectHealthcare-associated infections
dc.subjectMetagenomics
dc.subjectNanopore sequencing
dc.subject.dspaceBiology
dc.titleOptimizing surface sampling for healthcare pathogen detection: The effect of sample size and swabbing techniques on pathogen recovery
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