Study of genetic transformation efficiency via organogenesis and embryogenesis in eggplant (Solanum melongena L. cv. Embti): effects of co-culture, temperature and kanamycin and hygromycin-based selection procedures

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dc.contributor.authorPicoli, E. A. de T.
dc.contributor.authorBrommonschenkel, S. H.
dc.contributor.authorCecon, P. R.
dc.contributor.authorDa Silva, D. J. H.
dc.contributor.authorFári, M. G.
dc.contributor.authorOtoni, W. C.
dc.date.accessioned2021-06-28T09:58:20Z
dc.date.available2021-06-28T09:58:20Z
dc.date.issued2002-10-16
dc.description.abstractThe effects of kanamycin and hygromycin-based selection and co-culture temperature ranging from 22 to 28 °C upon eggplant transformation efficiency were evaluated. Both morphogenic pathways, somatic embryogenesis and organogenesis, were adopted using cotiledonary and hypocotyl explants, respectively. Somatic embryos were recovered in the presence of both antibiotics, although lesser escapes were observed in hygromycin-supplemented medium. Indeed, selection provided by this antibiotic was more efficient compared to kanamycin, nevertheless, shoot regeneration was not observed with hygromycin. Significant difference on the frequency of cotiledonary explants displaying callus (FEC) was observed as embryogenesis was concerned, although a higher number of embryos was observed in hygromycin selective media. The frequency of explants presenting callus (FEC), embryos (FEE) and shoots or buds (FERG) did not differ statistically for the tested co-culture temperatures, although higher regenerant number was observed at 24 °C.en
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dc.identifier.citationInternational Journal of Horticultural Science, Vol. 8 No. 3-4 (2002) , 15-23.
dc.identifier.doihttps://doi.org/10.31421/IJHS/8/3-4/355
dc.identifier.eissn2676-931X
dc.identifier.issn1585-0404
dc.identifier.issue3-4
dc.identifier.jatitleInt. j. hortic. sci.
dc.identifier.jtitleInternational Journal of Horticultural Science
dc.identifier.urihttps://hdl.handle.net/2437/314161en
dc.identifier.volume8
dc.languageen
dc.relationhttps://ojs.lib.unideb.hu/IJHS/article/view/355
dc.rights.accessOpen Access
dc.rights.ownerInternational Journal of Horticultural Science
dc.subjectSolanum melongenaen
dc.subjectgenetic transformationen
dc.subjectantibioticsen
dc.subjectco-culture temperatureen
dc.titleStudy of genetic transformation efficiency via organogenesis and embryogenesis in eggplant (Solanum melongena L. cv. Embti): effects of co-culture, temperature and kanamycin and hygromycin-based selection proceduresen
dc.typefolyóiratcikkhu
dc.typearticleen
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International Journal of Horticultural Science