Overexpression of two upstream phospholipid signaling genes improves cold stress response and hypoxia tolerance, but leads to developmental abnormalities in barley

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dc.contributor.authorGierczik, Krisztián
dc.contributor.authorSzékely, András
dc.contributor.authorAhres, Mohamed
dc.contributor.authorMarozsán-Tóth, Zsuzsanna
dc.contributor.authorVashegyi, Ildikó
dc.contributor.authorHarwood, Wendy
dc.contributor.authorTóth, Balázs
dc.contributor.authorGaliba, Gábor
dc.contributor.authorSoltész, Alexandra
dc.contributor.authorVágújfalvi, Attila
dc.contributor.statusPhD hallgatóhu_HU
dc.contributor.statusegyetemi oktató, kutatóhu_HU
dc.coverage.temporal2020.04.30.hu_HU
dc.date.accessioned2021-05-27T12:02:27Z
dc.date.available2021-05-27T12:02:27Z
dc.description.abstractPhosphatidylinositol transfer protein (PITP) and phosphatidylinositol 4-kinase (PI4K) are very upstream regulatory elements of the phospholipid signaling pathway in the signal transduction network. Unlike in animal systems, their role in stress signaling is poorly understood in plants. To study this area, PITP- and PI4K-overexpressing transgenic barley lines were developed. Morphological and developmental abnormalities were surveyed and characterized. It was revealed that the overexpression of the upstream signaling genes led to more phenotypic abnormalities than in other transgenic studies working with effector genes or even transcription factors. We hypothesize that this high level of abnormalities is the consequence of the modulation of the very upstream signal transduction pathway elements. On the other hand, we also revealed that overexpression of the PITP and PI4K genes increased stress tolerance during hypoxic cold stress, but not during salinity stress. Differences were also found in the level of frost tolerance between the transgenic overexpression plants and the recipient Golden Promise line. Molecular analysis showed that this improvement was not related to the most important cold responsive transcription factors, the CBF genes. We conclude that the transgenic method may be useful to prove the role of an upstream signaling element; however, due to the many developmental consequences that occur as side effects, it is a less advisable approach to achieve improved stress tolerance.hu_HU
dc.format.extent13hu_HU
dc.identifier.urihttp://hdl.handle.net/2437/310387
dc.language.isoenhu_HU
dc.subjectPhosphatidylinositol transfer proteinhu_HU
dc.subjectPhosphatidylinositol 4-kinasehu_HU
dc.subjectSignal transductionhu_HU
dc.subjectAbiotic stress tolerancehu_HU
dc.subjectMorphogenetic disordershu_HU
dc.subject.disciplinetudományterületek::növénytudományokhu_HU
dc.titleOverexpression of two upstream phospholipid signaling genes improves cold stress response and hypoxia tolerance, but leads to developmental abnormalities in barleyhu_HU
dc.typeidegen nyelvű peer review publikációhu_HU
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