At present, there is strong research interest in the nutritional quality and health benefits of food, which is supported by public awareness and an ongoing desire to improve our quality of life. Consequently, targeted modification of the FA profile of milk fat is desirable. The data obtained in this study shows there are differences in the amount of examined lipids in different milk samples. The polyunsaturated fatty acids are found in fewer amounts compared to MUFA and SFAs. Especially in Cow milk, the PUFAs are in lower amounts compared to the rest. Though both n-3 PUFAs and n-6 PUFAs are generally considered to have beneficial health effects, a high omega-6 fatty acid intake, and a high omega-6/omega-3 ratio are associated with weight gain in both animal and human studies, whereas a high omega-3 fatty acid intake decreases the risk for weight gain.