Identification of trypanosomes in skin and adipose tissue of white fulani & sokoto gudali cattle

dc.contributor.advisorBarta, Zoltán
dc.contributor.authorAsante, Enoch Ankomah
dc.contributor.departmentDE--Természettudományi és Technológiai Kar--Biológiai és Ökológiai Intézet
dc.date.accessioned2023-04-26T08:44:16Z
dc.date.available2023-04-26T08:44:16Z
dc.date.created2023-04-25
dc.description.abstractTrypanosomes are flagellated protozoan parasites that live in the blood, lymph and various tissues of their vertebrate hosts. They cause Trypanosomiasis and are transmitted cyclically by the tsetse flies of the genus Glossina or mechanically (non-cyclical) by the horse fly and other biting flies. The two types of trypanosomiasis are Human African Trypanosomiasis (HAT) which infect humans and Animal African Trypanosomiasis (AAT) which infect animals especially ruminants such as cattle. This study was done to identify the presence of trypanosome parasite in two breeds of cattle to determine the susceptibility of these breeds (White Fulani and Sokoto Gudali) and also the study was conducted due to the challenge in detecting trypanosome parasite in the blood by microscopy. The major trypanosomes that infect livestock are the T. congolense, T.vivax and T. brucei. All three species of trypanosomes will eventually cause death in their hosts unless treated and this increases the mortality and morbidity rate in agricultural animals. For the study, skin and fat samples from 20 cattle of two different breeds were obtained from an abattoir in Avenor which has a semi intensive farming system. Trypanosome DNA was detected using nested PCR targeting the tubulin gene cluster. From this study, 40% of samples from the Sokoto Gudali breed were positive while trypanosomes DNA were detected in 80% of the samples from White Fulani. Trypanosomes detected were suspected to be either T. brucei or T. congolense. The results suggest that trypanosomiasis is prevalent among cattle breeds in Avenor locality of Greater Accra with T. brucei as the predominant causal species of the disease, affecting both breeds but infection rate is higher in White Fulani than Sokoto Gudali.
dc.description.correctorLB
dc.description.courseBiology
dc.description.degreeBSc/BA
dc.format.extent50
dc.identifier.urihttps://hdl.handle.net/2437/350879
dc.language.isoen
dc.rights.accessHozzáférhető a 2022 decemberi felsőoktatási törvénymódosítás értelmében.
dc.subjectAnimal African Trypanosomiasis
dc.subjectPolymerase Chain Reaction
dc.subjectSokoto Gudali
dc.subjectWhite Fulani
dc.subjectGhana
dc.subjectSkin
dc.subjectAdipose tissue
dc.subject.dspaceDEENK Témalista::Biológiai tudományok::Evolúciós állattan
dc.titleIdentification of trypanosomes in skin and adipose tissue of white fulani & sokoto gudali cattle
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