Analysis of longevity in Holstein Friesian cattle using proteomic approaches

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dc.contributor.authorGulyás, Gabriella
dc.contributor.authorBéri, Béla
dc.contributor.authorJávor, András
dc.contributor.authorMárk, László
dc.contributor.authorCsősz, Éva
dc.contributor.authorPohóczky, Krisztina
dc.contributor.authorSoltész, Beáta
dc.contributor.authorKuti, Dániel
dc.contributor.authorCzeglédi, Levente
dc.date.accessioned2021-06-28T10:55:42Z
dc.date.available2021-06-28T10:55:42Z
dc.date.issued2012-07-31
dc.description.abstractThe aim of the present study was to determine marker proteins those are associated with functional longevity of dairy cattle. Holstein-Friesian cows were grouped based on their performance as follows: group 1) individuals with good longevity traits; group 2) short production life because of poor reproduction traits; group 3) short production life with low milk yield. Twelve individuals were sampled in each group, blood and milk samples were collected from cows. Blood samples were analysed with two dimensional polyacrylamide gel electrophoresis (2D PAGE), MALDI TOF/TOF and nanoLCMS/MS. The milk samples were analysed with MALDI TOF/TOF and nanoLC-MS/MS. Using the optimized gel based proteomic approach, we have succesfully separated 143 proteins in the group1, 139 proteins in the group2 and 136 proteins in the group3, but we could not find significant differences between groups in the expression pattern. Using MALDI TOF/TOF and nanoLC-IonTrap MS, we have found eleven protein sequences those were expressed only in the samples of good longevity group.en
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dc.identifier.citationActa Agraria Debreceniensis, No. 48 (2012) , 21-25
dc.identifier.doihttps://doi.org/10.34101/actaagrar/48/2447
dc.identifier.issn2416-1640
dc.identifier.issue48
dc.identifier.jatitleActa agrar. Debr.
dc.identifier.jtitleActa Agraria Debreceniensis
dc.identifier.urihttps://hdl.handle.net/2437/316106en
dc.languageen
dc.relationhttps://ojs.lib.unideb.hu/actaagrar/article/view/2447
dc.rights.accessOpen Access
dc.subjectlongevityen
dc.subjectHolstein-Friesianen
dc.subjectproteomicsen
dc.subject2D-PAGEen
dc.titleAnalysis of longevity in Holstein Friesian cattle using proteomic approachesen
dc.typefolyóiratcikkhu
dc.typearticleen
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