Bean tissue culture and genetic transformation with Agrobacterium

dc.contributor.authorEissa Ahmed, E.
dc.contributor.authorBisztray, Gy.
dc.contributor.authorVelich, I.
dc.date.accessioned2021-06-28T09:57:16Z
dc.date.available2021-06-28T09:57:16Z
dc.date.issued2000-02-23
dc.description.abstractIn this paper we report the establishment methods of a rapidly growing callus culture of Phaseolus vulgaris bean as well as the conditions required for a high level of transient gene expression using Agrobacterium-mediated transformation. A vector is containing both the lindan-resistance gene as a selectable marker, and GUS gene as a screenable marker. By using hypocotyl explant and vertical culture on B5 medium supplemented with 1 mg/1 kinetin- and 2,4-D 2 mg/1 and subcultured every 3-4 weeks, we can recommend to get a good and much callus from bean. This will help in introducing foreign DNA into callus cells. One strain of Agrobacterium carrying plasmid as vector for introducing foreign DNA into plant cells was used. At different concentrations of lindan; 3, 4 and 4.5 mg/I, the transformed Maxidor callus survived and grew over a period of 6 month and subcultured every 3-4 weeks, but the control callus died. Callus were assayed for GUS activity to confirm the expression of the GUS gene using the histochemical assay test. The GUS gene was also correctly expressed in callus cultures grown on 4mg/I lindan-selected medium, the typical blue colour in the histochemical assay using the X-gluc as substrate. But the control, non-transformed callus was not able to grow in the presence of lindan, neither showed a positive reaction in the in vitro assays.en
dc.formatapplication/pdf
dc.identifier.citationInternational Journal of Horticultural Science, Vol. 6 No. 1 (2000) , 32-35.
dc.identifier.doihttps://doi.org/10.31421/IJHS/6/1/62
dc.identifier.eissn2676-931X
dc.identifier.issn1585-0404
dc.identifier.issue1
dc.identifier.jatitleInt. j. hortic. sci.
dc.identifier.jtitleInternational Journal of Horticultural Science
dc.identifier.urihttps://hdl.handle.net/2437/313992en
dc.identifier.volume6
dc.languageen
dc.relationhttps://ojs.lib.unideb.hu/IJHS/article/view/62
dc.rights.accessOpen Access
dc.rights.ownerInternational Journal of Horticultural Science
dc.subjectPhaseolus vulgarisen
dc.subjectbean callusen
dc.subjectAgrobacteriumen
dc.subjectlindan resistanceen
dc.subjectGUS transformationen
dc.titleBean tissue culture and genetic transformation with Agrobacteriumen
dc.typefolyóiratcikkhu
dc.typearticleen
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