Nutritional and seasonal requirements for callus growth in Taxus baccata

A tétel rövid nézete
dc.contributor.authorBrunáková, K.
dc.contributor.authorBabincová, Z.
dc.contributor.authorCellárová, E.
dc.date.accessioned2021-06-28T09:57:47Z
dc.date.available2021-06-28T09:57:47Z
dc.date.issued2000-08-23
dc.description.abstractCallus cultures derived from young stems of two varieties of Taxus baccata cv. aureovariegata (genotype I) and Taxus baccata L. (genotype II, III) were induced. Gamborg's B5 medium was supplemented with different concentrations of auxin (2,4-D) in combination with cytokinins (kinetin or topolin) and with a phenolic-binding compound (PVP) to prevent callus darkening and growth inhibition. Stem explants displayed different responses to in vitro culture depending on plant genotype and on the season. Genetic variability was observed in the growth rate of calli initiated from all three genotypes of the same Taxus species. We found the best growth of callus cultures originated from the genotype ill in defined media. After the first subculture the majority of the cream-coloured primary callus turned brown and ceased its growth. However, the long-term culture was initiated.  en
dc.formatapplication/pdf
dc.identifier.citationInternational Journal of Horticultural Science, Vol. 6 No. 4 (2000) , 111-114.
dc.identifier.doihttps://doi.org/10.31421/IJHS/6/4/237
dc.identifier.eissn2676-931X
dc.identifier.issn1585-0404
dc.identifier.issue4
dc.identifier.jatitleInt. j. hortic. sci.
dc.identifier.jtitleInternational Journal of Horticultural Science
dc.identifier.urihttps://hdl.handle.net/2437/314073en
dc.identifier.volume6
dc.languageen
dc.relationhttps://ojs.lib.unideb.hu/IJHS/article/view/237
dc.rights.accessOpen Access
dc.rights.ownerInternational Journal of Horticultural Science
dc.subjecttaxolen
dc.subjectTaxus baccataen
dc.subjectcallogenesisen
dc.titleNutritional and seasonal requirements for callus growth in Taxus baccataen
dc.typefolyóiratcikkhu
dc.typearticleen
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International Journal of Horticultural Science (DE-journals)