Bottom-up analysis of commercial protein supplements using CE-MS

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dc.contributor.advisorGáspár, Attila
dc.contributor.advisorVishwakarma, Gayatri
dc.contributor.authorCliance, Saniga
dc.contributor.departmentDE--Természettudományi és Technológiai Kar--Kémiai Intézet
dc.date.accessioned2024-06-06T13:01:23Z
dc.date.available2024-06-06T13:01:23Z
dc.date.created2024-04-23
dc.description.abstractThe aim of the study is to focus on the analysis of commercial proteins and their composition with a bottom-up approach using capillary electrophoresis-mass spectrometry. There were eight different pea protein samples and the aim was to identify large number of proteins. We identified more than 200 proteins and 18 unique proteins were found with different protein id and mass in all samples.. This mainly includes storage proteins, which are essential proteins found in peas, such as vicilin, convicilin, legumin and their fragments. Then we compared the analytical performance of pea proteins with different lengths of bare fused silica and various injection volumes to get better results. We identified most of the similar proteins that could be identified in both lengths as having a high LogP value, but some were not also detected in specific lengths. Thus, we can say that, as the essential proteins that could be seen in peas, this could enhance plant-based proteins and used for commercial purpose.
dc.description.courseAnalytical Chemistry
dc.description.degreeMSc/MA
dc.format.extent45
dc.identifier.urihttps://hdl.handle.net/2437/371064
dc.language.isoen
dc.rights.accessHozzáférhető a 2022 decemberi felsőoktatási törvénymódosítás értelmében.
dc.subjectBottom-up analysis
dc.subjectCapillary electrophoresis -mass spectrometry
dc.subjectProteomics
dc.subjectCommercial protein supplements
dc.subject.dspaceChemistry
dc.titleBottom-up analysis of commercial protein supplements using CE-MS
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