Generation of CAR-T cell lines

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dc.contributor.advisorMedyouni, Ghofrane
dc.contributor.advisorHajdu, Péter Béla
dc.contributor.authorLima de Souza Pinto, Maria Eduarda
dc.contributor.departmentDE--Természettudományi és Technológiai Kar--Biotechnológiai Intézet
dc.date.accessioned2023-04-28T09:32:16Z
dc.date.available2023-04-28T09:32:16Z
dc.date.created2023
dc.description.abstractMany forms of hematological malignancies as well as solid tumours can be treated using cellular immunotherapy, such as CAR-T, a treatment using T cells that express an antibody-based chimeric antigen receptor targeting tumor antigen. Due to the current challenges related to CAR-T cell therapy, as well as to investigate the potential of third-generation CARs, this study aimed to generate CAR-T cells by retroviral transduction and its sorting based on sGFP expression though Fluorescence-Activated Cell Sorting (FACS). The results obtained with flow cytometry analysis demonstrated approximately 52% of transduction efficiency. The confocal microscopy was able to detect sGFP signal, assessing the cellular distribution of sGFP-tagged CARs. The transduced cells were sorted using Fluorescence-Activated Cell Sorting (FACS) method. At the end of the process, one tube was obtained containing Jurkat cells highly expressing sGFP, corresponding to 46% of the original population.
dc.description.correctorLB
dc.description.courseBiochemical Engineering
dc.description.degreeBSc/BA
dc.format.extent28
dc.identifier.urihttps://hdl.handle.net/2437/351202
dc.language.isoen
dc.rights.accessHozzáférhető a 2022 decemberi felsőoktatási törvénymódosítás értelmében.
dc.subjectCAR-T cells
dc.subjectFlow cytometry
dc.subjectRetroviral transduction
dc.subjectFluorescence-Activated Cell Sorting
dc.subject.dspaceDEENK Témalista::Biológiai tudományok::Immunológia
dc.titleGeneration of CAR-T cell lines
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