Genetic transformation of bean callus via Agrobacterium- mediated DNA transfer

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dc.contributor.authorEissa Ahmed, E.
dc.date.accessioned2021-06-28T09:58:40Z
dc.date.available2021-06-28T09:58:40Z
dc.date.issued2003-10-20
dc.description.abstractCallus cultures were induced from hypocotyl of young bean seedlings. Callus developed and maintenaned on B5 medium supplemented with 2mg/1 2,4-D and 1 mg/1 kinetin. The results demonstrate that A. tumefacins-mediated transformation is a convenient method to obtain transient gene expression in callus of bean. The results have shown that the bean callus co-cultivated with A. tumefaciens can be transformed to get heibicide Finale (glufosinate-ammonium) resistant GUS positive tissues. Southern blot analysis of transformed calli showed integration of gusA marker gene carried by a binary vector. Transformed calli were selected on herbicide containing media. Data of molecular analysis (Southern blotting) confirmed the insertion of gusA gene in the genome of herbicide resistant calli with bar gene. There are three evidences that calli are stable transformants: (1) herbicide resistance, (2) GUS activity which is indicative since the coding region containing an intron, (3) the results of Southern hybridization technique.en
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dc.identifier.citationInternational Journal of Horticultural Science, Vol. 9 No. 3-4 (2003) , 49-53.
dc.identifier.doihttps://doi.org/10.31421/IJHS/9/3-4/405
dc.identifier.eissn2676-931X
dc.identifier.issn1585-0404
dc.identifier.issue3-4
dc.identifier.jatitleInt. j. hortic. sci.
dc.identifier.jtitleInternational Journal of Horticultural Science
dc.identifier.urihttps://hdl.handle.net/2437/314211en
dc.identifier.volume9
dc.languageen
dc.relationhttps://ojs.lib.unideb.hu/IJHS/article/view/405
dc.rights.accessOpen Access
dc.rights.ownerInternational Journal of Horticultural Science
dc.subjectPhaseolus vulgarisen
dc.subjectbeanen
dc.subjectAgrobacteriumen
dc.subjecttransformationen
dc.subjectglufosinate-ammoniumen
dc.subjectGUSen
dc.titleGenetic transformation of bean callus via Agrobacterium- mediated DNA transferen
dc.typefolyóiratcikkhu
dc.typearticleen
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International Journal of Horticultural Science (DE-journals)