Szerző szerinti böngészés "Khangembam, Rojesh"

Megjelenítve 1 - 9 (Összesen 9)
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    A case report: sheep endoparasitism dynamics under semi-dry continental climate of Karcag, Hungary
    (2019) Tóth, Mariann; Khangembam, Rojesh; Farkas, Róbert; Oláh, János; Vass, Nóra; Monori, István
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    Point of care colourimetric and lateral flow LAMP assay for the detection of Haemonchus contortus in ruminant faecal samples
    (2021) Khangembam, Rojesh; Tóth, Mariann; Vass, Nóra; Várady, Marián; Czeglédi, Levente; Farkas, Róbert; Antonopoulos, Alistair
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    Preliminary Results of the Recombinase Polymerase Amplification Technique for the Detection of Haemonchus contortus from Hungarian Field Samples
    (2023) Khangembam, Rojesh; Vass, Nóra; Morrison, Alison; Melville, Lynsey A; Antonopoulos, Alistair; Czeglédi, Levente
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    Qualitative assessment of Haemonchus contortus infection in small ruminant flocks in Hungary: prevalence and diagnostic study
    Khangembam, Rojesh; Pálfyné Vass, Nóra; Állattenyésztési Tudományok Doktori Iskola; Debreceni Egyetem::Mezőgazdaság-, Élelmiszertudományi és Környezetgazdálkodási Kar
    Among the many gastro-intestinal nematode (GIN) parasites that can affect small ruminants, the family of Trichostrongylidae is very important and out of which Haemonchus contortus has established itself to be the most dangerous and clinically important species. A severely affected animal can harbour up to 5000 worms inside it and this can lead to the continual loss of about 250 ml of blood daily, which is fatal. A recent study in the EU estimated a production loss of 81% and 19% to treatment out of a total € 1.8 billion annual expenditure due to haemonchosis. Despite this, the specific diagnosis of the parasite, from the Trichostrongylidae family, is difficult using the conventional copromicroscopic tools wherein a correct diagnosis is crucial for sustainable control. Also, the parasite has been reported in almost all the EU nations however, very limited published reports are available for Hungary. The present study analysed n=16 farms located in 5 different counties in Eastern and Central Hungary. Out of these farms, n=14 were sheep farms; 1 goat farm and a village where roe deers were hunted. Faecal samples were collected from individual animals and the faecal egg count (FEC) data using the Mini-FLOTAC technique were obtained from another larger study. The present study found out n=12 sheep farms, the goat farm as well and the roe deer all had a positive result for H. contortus. Another aspect of the study was the design and development of two isothermal amplification tools as a proof of concept for a reliable farm site diagnosis of the parasite. Firstly, a Loop-mediated Isothermal Amplification (LAMP) and another LAMP-lateral flow (LAMP-LF) assay for the qualitative detection of H. contortus from the faecal samples of ruminant flocks were successfully designed. These two LAMP assays allow visual detection to the unaided eyes without compromising the specificity and sensitivity. The analytical sensitivity is determined to be 2.5 x 10-4 ng/μl of DNA template. Secondly, a Recombinase Polymerase Assay (RPA) technique was also successfully designed with an analytical sensitivity of 0.1 ng/μl DNA template. This study also would like to register two preliminary findings: i) that the post-amplification clean-up for the RPA amplicons could be eliminated without any significant loss in analytical sensitivity, and ii) a colourimetric change detection using some commonly DNA intercalating dyes (such as Eva Green and SYBR Green dyes) is possible. Besides these assays, the study also optimised a centrifuge-free crude DNA extraction method, adapted using the Fill-FLOTAC apparatus, a magnetic beads DNA extraction kit and a motorised micropestle for egg disintegration. This crude DNA extraction from the eggs ‘obtained’ from the Fill-FLOTAC apparatus is significant for a true farm-site molecular diagnostic kit. In conclusion, the study proved the presence of the H. contortus parasite in Hungary with preliminary gene sequencing findings using three isolates showing distinct lineages. Nevertheless, a more detailed epidemiological study, an in-depth phylogenetic analysis using sequencing of both ITS2 and nad4 genes and improvement in the isothermal assay by incorporating quantitative data are all highly recommended.
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    Recovery and confirmation of Haemonchus contortus from abomasal contents of roe deer (Capreolus capreolus) in Eastern-Hungary (Biharugra): A diagnostic case study
    (2023-06-05) Khangembam, Rojesh; Tóth, Mariann; Gulyas, Gabriella; Czeglédi, Levente; Vass, Nóra
    Gastrointestinal parasites are ubiquitous. They occur both in wild and domesticated animals. Among such parasites of veterinary importance is the trichostrongyle worms, out of which the Haemonchus contortus species is regarded as the most pathogenic one in the small ruminant industry. The occurrence of this parasite in the sheep flock is now very well documented and an established fact in Europe, although the parasite was original of the warmer climatic region. Studies on the cross-transmission of H. contortus between the wild and domesticated animals are also on the rise although the question of the direction of transmission is still debated. This is an important area that needs to be addressed as it could potentially contribute indirectly to mitigating anthelmintic resistance. Hungary also has reported its share of the occurrence of the parasite, mainly in the sheep flock and a certain population of roe deer. The study presented here is the preliminary results of a diagnostic case study that confirms the presence of H. contortus in wild ruminant deer species that are close to the domesticated sheep population.
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    Routine microscopy examination of faecal samples as a tool for detection of common gastrointestinal parasites: a preliminary report from two Hungarian farms
    (2023-06-05) Khangembam, Rojesh; Czeglédi, Levente; Vass, Nóra
    Gastrointestinal parasitism in ruminant animals is a cause of major economic loss incurred by the livestock industry. Regardless of the frequency of the adopted therapeutic and prophylactic deworming strategies, the parasitic burden in a farm should be assessed regularly. One of the most widely used techniques to do so is the microscopic faecal egg examination and faecal egg counting method. Despite the technique being almost a century old from its first adoption, the principle behind the newer techniques of faecal egg examination is the same. This technique is still being used in routine farm screening and monitoring gastrointestinal parasitic load and faecal egg count reduction testing to assess the anthelmintic efficacy of the drugs used. Thus, the tool remains a choice for preliminary screening for important parasites and the subsequent deworming strategy. Our study here was part of a larger survey on the treatment efficiency as well as a broad epidemiological study of the trichostrongyle parasites in Hungary. We present a preliminary report on the detection of common gastrointestinal parasites from two farms in Hungary, including a species-specific confirmatory microscopy for Haemonchus contortus eggs.