Acta Agraria Debreceniensis (DE-journals)

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https://hdl.handle.net/2437/271455
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Böngészés

Acta Agraria Debreceniensis (DE-journals) Tárgyszó szerinti böngészés "2D-PAGE"

Megjelenítve 1 - 2 (Összesen 2)
  • Nincs kép
    TételSzabadon hozzáférhető
    Analysis of longevity in Holstein Friesian cattle using proteomic approaches
    (2012-07-31) Gulyás, Gabriella; Béri, Béla; Jávor, András; Márk, László; Csősz, Éva; Pohóczky, Krisztina; Soltész, Beáta; Kuti, Dániel; Czeglédi, Levente
    The aim of the present study was to determine marker proteins those are associated with functional longevity of dairy cattle. Holstein-Friesian cows were grouped based on their performance as follows: group 1) individuals with good longevity traits; group 2) short production life because of poor reproduction traits; group 3) short production life with low milk yield. Twelve individuals were sampled in each group, blood and milk samples were collected from cows. Blood samples were analysed with two dimensional polyacrylamide gel electrophoresis (2D PAGE), MALDI TOF/TOF and nanoLCMS/MS. The milk samples were analysed with MALDI TOF/TOF and nanoLC-MS/MS. Using the optimized gel based proteomic approach, we have succesfully separated 143 proteins in the group1, 139 proteins in the group2 and 136 proteins in the group3, but we could not find significant differences between groups in the expression pattern. Using MALDI TOF/TOF and nanoLC-IonTrap MS, we have found eleven protein sequences those were expressed only in the samples of good longevity group.
  • Nincs kép
    TételSzabadon hozzáférhető
    Effect of selenium supplement on proteome of chicken liver
    (2012-12-16) Gulyás, Gabriella; Czeglédi, Levente; Prokisch, József; Jávor, András
    The aims of the present study were to optimize a two dimensional polyacrylamide gel electrophoresis method to chicken liver proteome and to determine the changes of protein expression caused by selenium. Twelve broiler chicken were used in this experiment. The selenium intake was 0,2 mg/kg in the control group (6 chicken) and 4,25 mg/kg in the experimental group (6 chicken). Using the optimized proteomic approach, we have succesfully separated 747 proteins in the experimental group and 741 proteins in the control group. We found six proteins, wich expressed only in the samples of experimental group. Further investigations need to determine these six proteins with mass spectrometry (MS) and look for the correlation between the physiological effects of selenium and the expression of these proteins.