In our long-term experiment we examined the effect on fertilization and crop rotation on winter wheat yield. The result obtained, showed that wheat varieties and fertilization strongly influence the yields of winter wheat in crop rotation system. In general, this research points out fervently that the yield of winter wheat is dependent on the relationship between different rotation systems, different genotypes of winter wheat and different nitrogen levels for winter wheat. The recommended combination of fertilizer dosage, forecrop rotation system and the different genotypes of winter wheat undoubtedly set the stage for further work to determine the best of the three genotypes for optimum and sustainable winter wheat production.