Primers designed for the detection of grapevine pathogens spreading with propagating material by quantitative real-time PCR

Dátum
Szerzők
Czotter, N.
Manduláné Farkas, E.
Lózsa, R.
Ember, I.
Szûcsné Varga, G.
Várallyay, É.
Szegedi, E.
Folyóirat címe
Folyóirat ISSN
Kötet címe (évfolyam száma)
Kiadó
University of Debrecen
Absztrakt
Leírás
Several grapevine pathogens are disseminated by propagating material as systemic, but latent infections. Their detection and identification have a basic importance in the production and handling of propagating stocks. Thus several sensitive and reliable diagnostic protocols mostly based on molecular techniques have been developed. Of these methods quantitative real-time PCR (q-PCR) has recently got an emerging importance. Here we collected primer data for the detection and identification of grapevine pathogens which are important in the production of propagating stocks by q-PCR. Additional novel techniques that use DNA amplification, hybridization and  sequencing are also briefly reviewed.
Kulcsszavak
bacteria, fungi, phytoplasmas, polymerase chain reaction, primer sequences, viroids, viruses, Vitis vinifera
Forrás