Aspergillus species are filamentous molds that are omnipresent in nature, developing in soil, on plants and on breaking down natural issue. Aspergillus fumigatus is the main cause of invasive aspergillosis which leads to fatal pulmonary diseases. Many susceptibility tests have been performed using different antifungal agents to prevent aspergillosis. The highly diversified variety of the included species, their antifungal susceptibility patterns and the seriousness of the pathology require quick and reliable strategies for identification. Amphotericin B is recommended to treat triazole-resistant strains of Aspergillus fumigatus. However, AMB possibly exerts its antifungal activity by oxidative injury rather than by an increase of membrane permeation. During the broth dilution method of Aspergillus fumigatus with antifungal agent, only the AMB stopped the growth of A. fumigatus, but the nucleoside analogue has not got any negative effect on the growth of A. fumigatus. The agent might be used as a nutrient and we expected the agent to show some effect on cellular level i.e. DNA, RNA, and cell membrane but the agent did not show any effect. Moreover, it also promoted growth in some wells. In the future, opportunities will exist to evaluate combination antifungal therapy as a viable treatment option for invasive aspergillosis.