Aminoglycoside antibiotics affect the in vitro morphogenic response of chrysanthemum and tobacco

A tétel rövid nézete
dc.contributor.authorTeixeira, Da Silva
dc.contributor.authorFukai, S.
dc.date.accessioned2021-06-28T09:58:19Z
dc.date.available2021-06-28T09:58:19Z
dc.date.issued2002-08-14
dc.description.abstractBroadly the success of genetic transformation of plants requires non-chimeric selection of transformed tissues and its subsequent regeneration. With rare exceptions, most plant transformation protocols still heavily utilize antibiotics for the selection of transgenic cells containing an antibiotic-degrading selectable marker gene. The morphogenic capacity of in vitro chrysanthemum and tobacco stem and leaf explants change with the addition of aminoglycoside antibiotics (AAs). Of 6 antibiotics tested, phytotoxicity occurred at 10-25 and 50-100 pgml -I in chrysanthemum and tobacco explants, respectively, depending on the size of the explant and the timing of application. The presence of light or darkness also had a significant effect. The use of transverse thin cell layers (tTCLs) in conjunction with high initial AA selection levels supported the greatest regeneration of transgenic material (adventitious shoots or callus) and the lowest number of escapes. Flow cytometric analyses demonstrate that regeneration can be predicted in both species, depending on the ploidy level of the callus. Endoreduplication was not observed in chrysanthemum, even at high AA levels, but occurred (8C or more) in tobacco callus, even at low AA concentrations (5-10 pgml -1 ). The higher the AA level, the greater the DNA degradation and the lower the 2C and 4C values.en
dc.formatapplication/pdf
dc.identifier.citationInternational Journal of Horticultural Science, Vol. 8 No. 2 (2002) , 93-104.
dc.identifier.doihttps://doi.org/10.31421/IJHS/8/2/346
dc.identifier.eissn2676-931X
dc.identifier.issn1585-0404
dc.identifier.issue2
dc.identifier.jatitleInt. j. hortic. sci.
dc.identifier.jtitleInternational Journal of Horticultural Science
dc.identifier.urihttps://hdl.handle.net/2437/314157en
dc.identifier.volume8
dc.languageen
dc.relationhttps://ojs.lib.unideb.hu/IJHS/article/view/346
dc.rights.accessOpen Access
dc.rights.ownerInternational Journal of Horticultural Science
dc.subjectaminoglycoside antibiotic (AA)en
dc.subjectDenclranthenza X grancliflorumen
dc.subjectNicotiana tabacunien
dc.subjectphytotoxicityen
dc.subjectpolyploidyen
dc.titleAminoglycoside antibiotics affect the in vitro morphogenic response of chrysanthemum and tobaccoen
dc.typefolyóiratcikkhu
dc.typearticleen
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