Transcriptional Profiling of the Candida auris Response to Exogenous Farnesol Exposure

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dc.contributor.authorJakab, Ágnes
dc.contributor.authorBalla, Noémi
dc.contributor.authorRagyák, Ágota
dc.contributor.authorNagy, Fruzsina
dc.contributor.authorKovács, Fruzsina
dc.contributor.authorSajtos, Zsófi
dc.contributor.authorTóth, Zoltán
dc.contributor.authorBorman, Andrew M.
dc.contributor.authorPócsi, István
dc.contributor.authorBaranyai, Edina
dc.contributor.authorMajoros, László
dc.contributor.authorKovács, Renátó
dc.contributor.authorJakab Ágnes (1987-) (biológus)
dc.contributor.authorKovács Renátó László (1987-) (molekuláris biológus, klinikai mikrobiológus)
dc.contributor.authorMajoros László (1966-) (szakorvos, klinikai mikrobiológus)
dc.contributor.authorTóth Zoltán (1990-) (molekuláris biológus)
dc.contributor.authorNagy Fruzsina (1991-) (molekuláris biológus)
dc.contributor.authorPócsi István (1961-) (vegyész)
dc.contributor.submitterdepBiotechnológiai Intézet -- 4401
dc.contributor.submitterdepOrvosi Mikrobiológiai Intézet -- 23
dc.contributor.submitterdepMolekuláris Biotechnológiai és Mikrobiológiai Tanszék -- 708
dc.contributor.submitterdepTTK
dc.contributor.submitterdepÁOK
dc.contributor.submitterdepDebreceni Egyetem
dc.date.accessioned2021-10-24T08:44:00Z
dc.date.available2021-10-24T08:44:00Z
dc.date.oa2021-11-09
dc.date.updated2021-10-24T08:44:00Z
dc.description.abstractThe antifungal resistance threat posed by Candida auris necessitates bold and innovative therapeutic options. Farnesol is a quorum-sensing molecule with a potential antifungal and/or adjuvant effect; it may be a promising candidate in alternative treatment regimens. To gain further insights into the farnesol-related effect on C. auris, genome-wide gene transcription analysis was performed using transcriptome sequencing (RNA-Seq). Farnesol exposure resulted in 1,766 differentially expressed genes. Of these genes, 447 and 304 genes with at least 1.5-fold increase or decrease in transcription, respectively, were selected for further investigation. Genes involved in morphogenesis, biofilm events (maturation and dispersion), gluconeogenesis, iron metabolism, and regulation of RNA biosynthesis showed downregulation, whereas those related to antioxidative defense, transmembrane transport, glyoxylate cycle, fatty acid β-oxidation, and peroxisome processes were upregulated. In addition, farnesol treatment increased the transcription of certain efflux pump genes, including MDR1, CDR1, and CDR2. Growth, measured by the change in the number of CFU, was significantly inhibited within 2 h of the addition of farnesol (5.8 × 107 ± 1.1 × 107 and 1.1 × 107 ± 0.3 × 107 CFU/ml for untreated control and farnesol-exposed cells, respectively) (P < 0.001). In addition, farnesol treatment caused a significant reduction in intracellular iron (152.2 ± 21.1 versus 116.0 ± 10.0 mg/kg), manganese (67.9 ± 5.1 versus 18.6 ± 1.8 mg/kg), and zinc (787.8 ± 22.2 versus 245.8 ± 34.4 mg/kg) (P < 0.05 to 0.001) compared to untreated control cells, whereas the level of cooper was significantly increased (274.6 ± 15.7 versus 828.8 ± 106.4 mg/kg) (P < 0.001). Our data demonstrate that farnesol significantly influences the growth, intracellular metal ion contents, and gene transcription related to fatty acid metabolism, which could open new directions in developing alternative therapies against C. auris.
dc.description.correctorLB
dc.identifier.citationmSphere. -[Epub ahead of print] : - (2021), p. -. -mSphere. -2379-5042
dc.identifier.doi10.1128/mSphere.00710-21
dc.identifier.issn2379-5042
dc.identifier.opachttp://webpac.lib.unideb.hu:8082/ebib/CorvinaWeb?action=cclfind&resultview=long&ccltext=idno+BIBFORM097539
dc.identifier.urihttp://hdl.handle.net/2437/323431
dc.identifier.urlhttps://journals.asm.org/doi/10.1128/mSphere.00710-21
dc.languageeng
dc.rights.accessopen access journal
dc.subject.otheridegen nyelvű folyóiratközlemény külföldi lapban
dc.tenderFK138462 -- OTKA
dc.tenderEFOP-3.6.3-VEKOP-16-2017-00009 -- EFOP
dc.tenderEFOP-3.6.1-16-2016-00022 -- EFOP
dc.titleTranscriptional Profiling of the Candida auris Response to Exogenous Farnesol Exposure
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