During the 2013–2014 period, mosquitoes of the Lake Balaton region were collected and examined for the presence of West Nile virus (WNV). Our test area contained biting mosquito habitats of considerable expanse and variety, as well as large numbers of presently residing or migrating bird fauna. Specimens of five potential vector species of WNV (Anopheles maculipennis s.l., Aedes vexans, Coquillettidia richiardii, Culex p. pipiens, Culex modestus) were involved in the virological studies. Our investigation consisted of 4,197 mosquito specimens. The RNA of WNV was not detected by qRT-PCR in any of the mosquito pools. We concluded that during the above mentioned time period there was not WNV activity around Lake Balaton. Although, during the same time period there were West Nile encephalitis cases found in the Eastern and Central part of the country, precisely 31 and 11 cases.