Callus induction on standard type Cymbidium cultivars
Fájlok
Dátum
2000-08-23
Szerzők
Folyóirat címe
Folyóirat ISSN
Kötet címe (évfolyam száma)
Kiadó
Absztrakt
Tissue cultured Cymbidium PLBs (protocormlike body) were used as starting material to induce embryogenic callus which could serve as objects of genetic transformation. We obtained callus using two methods. The first method was culturing the PLB segments for one month in liquid MS medium in the presence of 0.5 mg/1 benzyladenine and 0.05 mg/1 naphtylacetic acid followed by cultivation on the same composition solid medium with 0.5 g/l activated charcoal for an additional month. Callus formation was observed on 30% of the explants. The second way was to propagate the PLB segments on solid MS medium supplemented with 1 mg/1 thidiazuron. In these cultures we also observed callus formation on 20% of the explants.
Leírás
Kulcsszavak
Cymbidium hybrida, micropropagation, callus induction, benzyladenine, naphtylacetic acid, thidiazuron
Forrás
International Journal of Horticultural Science, Vol. 6 No. 4 (2000)
, 108-110.