Callus induction on standard type Cymbidium cultivars

A tétel rövid nézete
dc.creatorJánvári, L.
dc.creatorBisztray, Gy.
dc.creatorFüzesi, T.
dc.creatorVelich, I.
dc.date2000-08-23
dc.date.accessioned2021-06-28T09:57:47Z
dc.date.available2021-06-28T09:57:47Z
dc.descriptionTissue cultured Cymbidium PLBs (protocormlike body) were used as starting material to induce embryogenic callus which could serve as objects of genetic transformation. We obtained callus using two methods. The first method was culturing the PLB segments for one month in liquid MS medium in the presence of 0.5 mg/1 benzyladenine and 0.05 mg/1 naphtylacetic acid followed by cultivation on the same composition solid medium with 0.5 g/l activated charcoal for an additional month. Callus formation was observed on 30% of the explants. The second way was to propagate the PLB segments on solid MS medium supplemented with 1 mg/1 thidiazuron. In these cultures we also observed callus formation on 20% of the explants.
dc.formatapplication/pdf
dc.identifierhttps://ojs.lib.unideb.hu/IJHS/article/view/236
dc.identifier10.31421/IJHS/6/4/236
dc.identifier.urihttp://hdl.handle.net/2437/314072
dc.languageeng
dc.publisherUniversity of Debrecen
dc.relationhttps://ojs.lib.unideb.hu/IJHS/article/view/236/235
dc.rightsCopyright (c) 2018 International Journal of Horticultural Science
dc.sourceInternational Journal of Horticultural Science; Vol. 6 No. 4 (2000); 108-110.
dc.source2676-931X
dc.source1585-0404
dc.source10.31421/IJHS/6/4
dc.subjectCymbidium hybrida
dc.subjectmicropropagation
dc.subjectcallus induction
dc.subjectbenzyladenine
dc.subjectnaphtylacetic acid
dc.subjectthidiazuron
dc.titleCallus induction on standard type Cymbidium cultivars
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
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International Journal of Horticultural Science