Test of the utility of apple retrotransposon insertion patterns for molecular identification of 'Jonathan' somatic mutants

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dc.contributor.authorGalli, Zs.
dc.contributor.authorWichmann, B.
dc.contributor.authorKiss, E.
dc.contributor.authorSzabó, T.
dc.contributor.authorHeszky, L.
dc.date.accessioned2021-06-28T10:00:38Z
dc.date.available2021-06-28T10:00:38Z
dc.date.issued2008-05-19
dc.description.abstractUp until today, apple sport mutants proved to be indistinguishable from each other and their progenitors at the molecular level using random amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP) and simple sequence repeat (SSR) marker techniques. This is not surprising, since the genomes of these somatic mutants differ only in one or a few small regions that affect economically important characteristics, such as improved fruit colour, size, or flavour. In most cases, these genome differences are probably caused by retrotransposons which are able to convert their RNA transcripts to DNA with reverse transcriptase enzyme prior to reinsertion, but unable to leave the genome and infect other cells. Retrotransposon insertions can alter the expression of other genes and/or the structure of encoded proteins. The sequence-specific amplified polymorphism (S-SAP) technique is capable of revealing the genetic distribution of retrotransposable elements over the whole genome. The present study used this approach to try to characterize and distinguish 'Jonathan' somatic mutants via fingerprinting, which is an unsolved problem.en
dc.formatapplication/pdf
dc.identifier.citationInternational Journal of Horticultural Science, Vol. 14 No. 3 (2008) , 7-10.
dc.identifier.doihttps://doi.org/10.31421/IJHS/14/3/794
dc.identifier.eissn2676-931X
dc.identifier.issn1585-0404
dc.identifier.issue3
dc.identifier.jatitleInt. j. hortic. sci.
dc.identifier.jtitleInternational Journal of Horticultural Science
dc.identifier.urihttps://hdl.handle.net/2437/314503en
dc.identifier.volume14
dc.languageen
dc.relationhttps://ojs.lib.unideb.hu/IJHS/article/view/794
dc.rights.accessOpen Access
dc.rights.ownerInternational Journal of Horticultural Science
dc.subjectapple DNA fingerprintingen
dc.subjectsequence-specific amplified polymorphism (S-SAP)en
dc.subjectbud mutationen
dc.titleTest of the utility of apple retrotransposon insertion patterns for molecular identification of 'Jonathan' somatic mutantsen
dc.typefolyóiratcikkhu
dc.typearticleen
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