Test of the utility of apple retrotransposon insertion patterns for molecular identification of 'Jonathan' somatic mutants

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dc.creatorGalli, Zs.
dc.creatorWichmann, B.
dc.creatorKiss, E.
dc.creatorSzabó, T.
dc.creatorHeszky, L.
dc.date2008-05-19
dc.date.accessioned2021-06-28T10:00:38Z
dc.date.available2021-06-28T10:00:38Z
dc.descriptionUp until today, apple sport mutants proved to be indistinguishable from each other and their progenitors at the molecular level using random amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP) and simple sequence repeat (SSR) marker techniques. This is not surprising, since the genomes of these somatic mutants differ only in one or a few small regions that affect economically important characteristics, such as improved fruit colour, size, or flavour. In most cases, these genome differences are probably caused by retrotransposons which are able to convert their RNA transcripts to DNA with reverse transcriptase enzyme prior to reinsertion, but unable to leave the genome and infect other cells. Retrotransposon insertions can alter the expression of other genes and/or the structure of encoded proteins. The sequence-specific amplified polymorphism (S-SAP) technique is capable of revealing the genetic distribution of retrotransposable elements over the whole genome. The present study used this approach to try to characterize and distinguish 'Jonathan' somatic mutants via fingerprinting, which is an unsolved problem.
dc.formatapplication/pdf
dc.identifierhttps://ojs.lib.unideb.hu/IJHS/article/view/794
dc.identifier10.31421/IJHS/14/3/794
dc.identifier.urihttp://hdl.handle.net/2437/314503
dc.languageeng
dc.publisherUniversity of Debrecen
dc.relationhttps://ojs.lib.unideb.hu/IJHS/article/view/794/792
dc.rightsCopyright (c) 2018 International Journal of Horticultural Science
dc.sourceInternational Journal of Horticultural Science; Vol. 14 No. 3 (2008); 7-10.
dc.source2676-931X
dc.source1585-0404
dc.source10.31421/IJHS/14/3
dc.subjectapple DNA fingerprinting
dc.subjectsequence-specific amplified polymorphism (S-SAP)
dc.subjectbud mutation
dc.titleTest of the utility of apple retrotransposon insertion patterns for molecular identification of 'Jonathan' somatic mutants
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
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International Journal of Horticultural Science