Prevalence and Genomic Epidemiology of MultiDrug-Resistant Bacteria in Dragonfly Larvae in Water Bodies

A tétel rövid nézete
dc.contributor.advisorKardos , Gábor
dc.contributor.advisordeptDebreceni Egyetem::Metagenomikai Intézet
dc.contributor.authorQuinga Nasimba, Mayra Vanessa
dc.contributor.departmentDE--Általános Orvostudományi Kar
dc.date.accessioned2023-11-20T09:35:02Z
dc.date.available2023-11-20T09:35:02Z
dc.date.created2023-06-07
dc.description.abstractMultidrug resistant Gram-negative bacteria are a public health concern worldwide. Resistance is not exclusively a problem of human medicine; it is spread to the environment, including water bodies, for example, due to the influx of human wastewater. This study investigates the prevalence of MDR bacteria in Hajdú-Bihar county in the river Berettyó at its entry to Hungary (47.25276, 21.83794). To understand the occurrence of MDR bacteria in small aquatic invertebrates as potential aquatic reservoirs of MDR bacteria, the presence of these in banded demoiselle (Calopteryx splendens) larvae was analyzed. Larvae were killed and MDR bacteria were enriched in MH broth supplemented with 2 mg/l cefotaxime then cultured on eosin methylene blue agar with 2 mg/l cefotaxime to look for MDR Enterobacterales. Recovered isolates were identified at the species level, and antibiotic susceptibility testing was performed. Isolates proved to be MDR bacteria and were subjected to third-generation whole genome sequencing. Based on their whole genomes, the resistome, MLST and cgMLST type were determined to assess the genomic epidemiology of the isolates. The prevalence of MDR Enterobacterales was 19/50 (38%); three Citrobacter freundii, nine Escherichia coli, five Klebsiella pneumoniae, three Morganella morganii and one Raoultella planticola were isolated. Of these, the four K. pneumoniae sequenced were 1 ST 147 carrying blaCTX-M15 and QnrB17, and 3 ST1119 carrying blaCTX-M15, sul1 and aac(3)-IIe). The 9 E. coli were 1 ST131 carrying blaCTX-M15; 1 further ST131 carrying blaCTX-M15 and blaCTX-M27; 2 ST182 of the same cgMLST type (148002) carrying blaCTX-M3 and blaCTX-M15, or blaCTX-M3; 2 ST609 of the same cgMLST type (180278) carrying blaCTX-M65 and qnrS1; 1 ST744 carrying blaCTX-M3 and blaCTX-M55 on two different plasmids as well as 2 isolates of unidentified STs. High prevalence of MDR bacteria including strains of potential human (ST1119 K. pneumoniae, ST131 E. coli) and veterinary (ST744 E. coli) interest points to the role of rivers and invertebrates inhabiting them as reservoirs and vehicles of transboundary spread of multi-resistance as highlighted in the One Health concept.
dc.description.coursemolekuláris biológia
dc.description.courselangangol
dc.description.coursespecImmunológia, sejt- és mikrobiológia
dc.description.degreeMSc/MA
dc.format.extent34
dc.identifier.urihttps://hdl.handle.net/2437/360926
dc.language.isoen
dc.rights.accessHozzáférhető a 2022 decemberi felsőoktatási törvénymódosítás értelmében.
dc.subjectMultidrug resistance; cgMLST; water bodies; ESBL; E.coli ST131, E.coli ST744, K. pneumoniae ST1119.
dc.subject.dspaceDEENK Témalista::Biológiai tudományok::Molekuláris biológia
dc.subject.dspaceDEENK Témalista::Biológiai tudományok::Mikrobiológia
dc.titlePrevalence and Genomic Epidemiology of MultiDrug-Resistant Bacteria in Dragonfly Larvae in Water Bodies
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