Fine needle aspiration cytology (FNAC) is a simple, economical, and minimally invasive technique for evaluating both superficial and deep-seated lesions, especially valuable in oncological diagnostics when combined with immunohistochemistry. Despite its effectiveness, false-negative results remain a concern, influenced by factors such as slide number and adequacy of cellular material. While increasing the number of passes may improve diagnostic yield, it also increases procedure time, patient discomfort, and potential adverse events. This study, conducted at the University of Debrecen on 45 randomly selected cases, compared the first pass (Slide A) and second pass (Slide B) specimens, each graded on a 1–5 quality scale. Results showed that over 90% of cases achieved a conclusive diagnosis from a single pass, reducing the necessity for multiple passes. These findings highlight FNAC as a cost-effective, safe, and efficient outpatient diagnostic procedure.