HPLC Analysis of Polyphenols Derived from Hungarian Aszú from Tokaj Wine Region and Its Effect on Inflammation in an In Vitro Model System of Endothelial Cells

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dc.contributor.authorMarkovics, Arnold
dc.contributor.authorCsige, László
dc.contributor.authorSzőllősi, Erzsébet
dc.contributor.authorMatyi, Hajnalka
dc.contributor.authorLukács, Andrea Diána
dc.contributor.authorPerez, Nóra Réka
dc.contributor.authorBacsó, Zsófia Réka
dc.contributor.authorStündl, László
dc.contributor.authorRemenyik, Judit
dc.contributor.authorBiró, Attila
dc.contributor.authorMarkovics Arnold (1988-) (molekuláris biológus)
dc.contributor.authorSzőllősi Erzsébet (1983-) (környezetkutató-ökológus)
dc.contributor.authorMatyi Hajnalka (2023-) (xxx)
dc.contributor.authorLukács Andrea Diána (2023-) (xxx)
dc.contributor.authorPerez Nóra Réka (2023-) (xxx)
dc.contributor.authorStündl László (1970-) (agrármérnök)
dc.contributor.authorGálné Remenyik Judit (1965-) (vegyész)
dc.contributor.authorBíró Attila (2023-) (xxx)
dc.contributor.submitterdepÉlelmiszertechnológiai Intézet -- 4061
dc.contributor.submitterdepMÉK
dc.contributor.submitterdepDebreceni Egyetem
dc.date.accessioned2023-07-24T12:56:26Z
dc.date.available2023-07-24T12:56:26Z
dc.date.oa2023-08-01
dc.date.updated2023-07-24T12:56:26Z
dc.description.abstractMany studies have been published in recent years regarding the fact that moderate wine consumption, as a part of a balanced diet can have a beneficial effect on human health. The bio- logically active components of wine continue to be the subject of intense research today. In this study, the bioactive molecules of Hungarian aszú from the Tokaj wine region were analyzed using high-performance liquid chromatography (HPLC) and investigated in an in vitro model system of endothelial cells induced by bacterial-derived lipopolysaccharide. The HPLC measurements were performed on a reversed phased column with gradient elution. The non-cytotoxic concentration of the active substance was determined based on 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyltetrazolium bromide (MTT)-, apoptosis, and necrosis assays. The antioxidant effect of the extract was determined by evaluating its ability to eliminate ROS. The expressions of the interleukin-(IL)1α, IL1-β, IL-6, and IL-8 pro-inflammatory cytokines and nitric oxide synthase (eNOS) at the mRNA level were evaluated using a quantitative polymerase chain reaction (qPCR). We found that the lipopolysaccharides (LPS)- induced increases in the expressions of the investigated cytokines were significantly suppressed by Hungarian aszú extract, excluding IL-6. In our experimental setup, our treatment had a positive effect on the eNOS expression, which was impaired as a result of the inflammatory manipulation. In our experimental model, the Hungarian aszú extract decreased the LPS-induced increases in the expression of the investigated cytokines and eNOS at the mRNA level, which presumably had a positive effect on the endothelial dysfunction caused by inflammation due to its strong antioxidant and anti-inflammatory effects. Collectively, this research contributes to a more thorough understanding of the bioactive molecules of aszú from the Tokaj wine region.
dc.description.correctorSZKM
dc.identifier.citationInternational Journal Of Molecular Sciences. -24 : 7 (2023), p. 1-14. -Int. J. Mol. Sci. -1422-0067
dc.identifier.doi10.3390/ijms24076124
dc.identifier.issn1422-0067
dc.identifier.opachttps://ebib.lib.unideb.hu/ebib/CorvinaWeb?action=cclfind&resultview=long&ccltext=idno+BIBFORM113646
dc.identifier.urihttps://hdl.handle.net/2437/357819
dc.identifier.urlhttps://www.mdpi.com/1422-0067/24/7/6124
dc.languageeng
dc.rights.accessOpen access journal
dc.rights.ownerSzerző
dc.subject.otheridegen nyelvű folyóiratközlemény külföldi lapban
dc.titleHPLC Analysis of Polyphenols Derived from Hungarian Aszú from Tokaj Wine Region and Its Effect on Inflammation in an In Vitro Model System of Endothelial Cells
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