Variation-Based Binding Affinity Analysis of the Human CTCF Transcription Factor

A tétel rövid nézete
dc.contributor.advisorBarta, Endre
dc.contributor.advisorKontra, Levente
dc.contributor.advisordeptDebreceni Egyetem::Általános Orvostudományi Kar::Biokémiai és Molekuláris Biológiai Intézet
dc.contributor.authorSakif, Agshin
dc.contributor.departmentDE--Általános Orvostudományi Kar
dc.contributor.opponentKónya, Zoltán
dc.contributor.opponentdeptDebreceni Egyetem::Általános Orvostudományi Kar::Orvosi Vegytani Intézet
dc.date.accessioned2023-06-17T10:44:31Z
dc.date.available2023-06-17T10:44:31Z
dc.date.created2023
dc.description.abstractUnlike other transcription factors, CTCF recognizes a longer core motif region and possesses multiple Zinc finger domains for reading additional sequences around the motif. CTCF's role in providing anchor sites for cohesin rings, which create DNA loops, is highlighted as crucial for gene expression regulation. We utilized the human ChIPSummitDB database, containing extensive data on CTCF binding sites, to conduct the study. We collected and analyzed ChIP-seq experiments, developing pipelines to automatically analyze raw sequencing data and identify variations in CTCF binding sites. The study reveals that specific bases in the CTCF motif, particularly the 2nd and 7th positions, exhibit higher frequencies of reference alleles due to their high binding affinity.
dc.description.correctorLB
dc.description.coursemolekuláris biológia
dc.description.courselangangol
dc.description.coursespecBiokémia-genomika
dc.description.degreeMSc/MA
dc.format.extent29
dc.identifier.urihttps://hdl.handle.net/2437/355748
dc.language.isoen
dc.rights.accessHozzáférhető a 2022 decemberi felsőoktatási törvénymódosítás értelmében.
dc.subjectTranscription Factor
dc.subjectConsensus Motif
dc.subjectCTCF
dc.subjectTranscription Factor Binding Site
dc.subjectAllele Frequency
dc.subject.dspaceDEENK Témalista::Biológiai tudományok::Genetika
dc.titleVariation-Based Binding Affinity Analysis of the Human CTCF Transcription Factor
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Theses (Faculty of Medicine)