Formulation and Investigation of CK2 Inhibitor-Loaded Microparticles.

Abstract

This thesis presents a well-structured and insightful investigation into the formulation of CK2 inhibitor-loaded microparticles using sodium alginate microbeads. It successfully demonstrates how encapsulating the drug enhances its bioavailability, stability, antioxidant activity, and cytotoxic effect against cancer cells, especially with the aid of Transcutol HP as a solubilizer. The methodology is clear and thorough, with detailed descriptions of formulation, testing, and analysis techniques such as swelling behavior, encapsulation efficiency, dissolution assays, and MTT cytotoxicity tests. Results show that microbeads containing Transcutol HP outperformed other formulations in drug release and antioxidant capacity. The use of the Caco-2 cell line adds validity to the in vitro findings, and the discussion effectively ties together the therapeutic potential of CK2 inhibitors with the advantages of controlled drug delivery systems. Overall, the thesis makes a valuable contribution to pharmaceutical technology and targeted cancer therapy research.