This thesis discusses 3D printing in the field of regenerative medicine, with the greatest emphasis on its application within dentistry and maxillofacial surgery. Tissue engineering by 3D printing based on the use of human stem cells has shown a promising expansion for the restoration of dental and oro-facial tissues. The practice of using stem cells in regenerative dentistry provides a large amplitude of possible applications compared to synthetic tissues. Hydrogels – the printable bioink has proven to be a favourable material for the printing of live and functional tissues.