Down-regulation of ethylene production in carnation (Dianthus Caryphyllus L.) by an apple derived ACC-cDNA
Fájlok
Dátum
Szerzők
Veres, A.
Kis, E.
Tóth, E.
Tóth, Á.
Heszky, L.
Folyóirat címe
Folyóirat ISSN
Kötet címe (évfolyam száma)
Kiadó
University of Debrecen
Absztrakt
Leírás
Transgenic carnations were produced with an apple derived antisense ACC-synthase cDNA. Transgenic carnation regenerants were potted in glasshouse. All transformed plants showed normal growth and were true-to-type. Ethylene production — measured at full opening stage — lowered by 30-60 %, no plant with 100 % decrease was identified. The vase-life has been observed for 5 years. 38 % of the transformant carnations showed a higher a relative value in days by more than 2 days to 6 days. Twenty six plants were found exhibiting the most marked alterations in the tested trait. In these plants ethylene production decreased by 37-67 %, they have longer vase-life (by 4 days or more). Since the fragrance variety 'Bíbor' was the plant material for genetic modification of vase-life, this trait has been conserved after transformation in spite of the fact that the position of transgene integration cannot be directed.
Kulcsszavak
ACC-synthase, MdACS2, transgenic carnation, vase-life