Neuronal Differentiation of mouse embryonic stem cells

A tétel rövid nézete
dc.contributor.advisorSzatmári, István
dc.contributor.advisordeptBiochemistry and Molecular Biologyhu_HU
dc.contributor.authorAgyare, Eunice
dc.contributor.departmentDE--Általános Orvostudományi Karhu_HU
dc.contributor.opponentPolgár, Zsuzsanna
dc.contributor.opponentdeptOrvosi Vegytani Intézethu_HU
dc.date.accessioned2019-06-21T09:32:03Z
dc.date.available2019-06-21T09:32:03Z
dc.date.created2019-06-13
dc.description.abstractThe major goal of this study is to differentiate mouse ESCs into neural progenitor cells (NPCs) and investigate the inducibility of a reporter transgene during this neuronal differentiation. I observed that this particular EGFP (enhanced green fluorescence protein) carrying ESC clone (C8) exhibited a very strong transgene inducibility comparing with the other EGFP clones and also this cell clone retained its transgene inducibility upon neural differentiation: 98% of the drug-induced cells were GFP positive after 6 day of differentiation. In addition, I observed that these ESC derived differentiated cells were also able to form neuronal networks and they expressed neural specific genes (Pax6 and Neurog2).hu_HU
dc.description.correctorLB
dc.description.coursemolekuláris biológiahu_HU
dc.description.courselangangolhu_HU
dc.description.coursespecBiokémia-genomikahu_HU
dc.description.degreeMSc/MAhu_HU
dc.format.extent33hu_HU
dc.identifier.urihttp://hdl.handle.net/2437/269914
dc.language.isoenhu_HU
dc.subjectBMP Bone Morphogenetic Proteins
dc.subjectbFGF Basic Fibroblast Growth Factor
dc.subjectCNS Central Nervous System
dc.subjectDOX Doxycycline
dc.subjectDMEM Dulbecco's Modified Eagle Medium
dc.subjectDMSO Dimethyl Sulfoxide
dc.subjectEGFP Green Fluorescence Protein
dc.subjectESCs Embryonic Stem cells
dc.subjectFACS Fluorescence Activated Cell Sorting
dc.subjectFBS Fetal Bovine Serum
dc.subjectFGF Fibroblast Growth Factor
dc.subjectGFAP Glial Fibrillary Acidic Protein
dc.subjectG418 Genetic
dc.subjectHSCs Hematopoietic Stem Cells
dc.subjecthESCs Human Embryonic Stem Cells
dc.subjectICM Inner Cell Mass
dc.subjectiPSCs Induced Pluripotent Stem Cells
dc.subjectIKMC International Knockout Mouse Consortium
dc.subjectKSR Knockout Serum Replacement
dc.subjectLIF Leukemia Inhibitory Factor
dc.subjectmESCs Mouse Embryonic Stem Cells
dc.subjectMEF Mouse Embryonic Fibroblast
dc.subjectN2B27 Neurobasal plus B27 supplement
dc.subjectNPCs Neuro Progenitor Cells
dc.subjectPBS Phosphate Buffered Saline
dc.subjectQ- PCR Quantitative Polymerase Chain Reaction
dc.subjectRGPs Radial Glial Progenitor
dc.subjectTA Transactivator
dc.subject.dspaceDEENK Témalista::Biológiai tudományokhu_HU
dc.titleNeuronal Differentiation of mouse embryonic stem cellshu_HU
Fájlok
Gyűjtemények
Theses (Faculty of Medicine)