Neuronal Differentiation of mouse embryonic stem cells
Absztrakt
The major goal of this study is to differentiate mouse ESCs into neural progenitor cells (NPCs) and investigate the inducibility of a reporter transgene during this neuronal differentiation. I observed that this particular EGFP (enhanced green fluorescence protein) carrying ESC clone (C8) exhibited a very strong transgene inducibility comparing with the other EGFP clones and also this cell clone retained its transgene inducibility upon neural differentiation: 98% of the drug-induced cells were GFP positive after 6 day of differentiation. In addition, I observed that these ESC derived differentiated cells were also able to form neuronal networks and they expressed neural specific genes (Pax6 and Neurog2).
Leírás
Kulcsszavak
BMP Bone Morphogenetic Proteins, bFGF Basic Fibroblast Growth Factor, CNS Central Nervous System, DOX Doxycycline, DMEM Dulbecco's Modified Eagle Medium, DMSO Dimethyl Sulfoxide, EGFP Green Fluorescence Protein, ESCs Embryonic Stem cells, FACS Fluorescence Activated Cell Sorting, FBS Fetal Bovine Serum, FGF Fibroblast Growth Factor, GFAP Glial Fibrillary Acidic Protein, G418 Genetic, HSCs Hematopoietic Stem Cells, hESCs Human Embryonic Stem Cells, ICM Inner Cell Mass, iPSCs Induced Pluripotent Stem Cells, IKMC International Knockout Mouse Consortium, KSR Knockout Serum Replacement, LIF Leukemia Inhibitory Factor, mESCs Mouse Embryonic Stem Cells, MEF Mouse Embryonic Fibroblast, N2B27 Neurobasal plus B27 supplement, NPCs Neuro Progenitor Cells, PBS Phosphate Buffered Saline, Q- PCR Quantitative Polymerase Chain Reaction, RGPs Radial Glial Progenitor, TA Transactivator